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Behavioral and Brain Functions
Published in: Behavioral and Brain Functions 1/2017

Open Access 01-12-2017 | Research

Effects of GABAB receptors in the insula on recognition memory observed with intellicage

Authors: Nan Wu, Feng Wang, Zhe Jin, Zhen Zhang, Lian-Kun Wang, Chun Zhang, Tao Sun

Published in: Behavioral and Brain Functions | Issue 1/2017

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Abstract

Background

Insular function has gradually become a topic of intense study in cognitive research. Recognition memory is a commonly studied type of memory in memory research. GABABR has been shown to be closely related to memory formation. In the present study, we used intellicage, which is a new intelligent behavioural test system, and a bilateral drug microinjection technique to inject into the bilateral insula, to examine the relationship between GABABR and recognition memory.

Methods

Male Sprague–Dawley rats were randomly divided into control, Sham, Nacl, baclofen and CGP35348 groups. Different testing procedures were employed using intellicage to detect changes in rat recognition memory. The expression of GABABR (GB1, GB2) in the insula of rats was determined by immunofluorescence and western blotting at the protein level. In addition, the expression of GABABR (GB1, GB2) was detected by RT-PCR at the mRNA level.

Results

The results of the intellicage test showed that recognition memory was impaired in terms of position learning, punitive learning and punitive reversal learning by using baclofen and CGP35348. In position reversal learning, no significant differences were found in terms of cognitive memory ability between the control groups and the CGP and baclofen groups. Immunofluorescence data showed GABABR (GB1, GB2) expression in the insula, while data from RT-PCR and western blot analysis demonstrated that the relative expression of GB1 and GB2 was significantly increased in the baclofen group compared with the control groups. In the CGP35348 group, the expression of GB1 and GB2 was significantly decreased, but there was no significant difference in GB1 or GB2 expression in the control groups.

Conclusions

GABABR expression in the insula plays an important role in the formation of recognition memory in rats.
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Metadata
Title
Effects of GABAB receptors in the insula on recognition memory observed with intellicage
Authors
Nan Wu
Feng Wang
Zhe Jin
Zhen Zhang
Lian-Kun Wang
Chun Zhang
Tao Sun
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Behavioral and Brain Functions / Issue 1/2017
Electronic ISSN: 1744-9081
DOI
https://doi.org/10.1186/s12993-017-0125-4

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