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Behavioral and Brain Functions
Published in: Behavioral and Brain Functions 1/2017

Open Access 01-12-2017 | Research

Interaction between cytochrome P450 2A6 and Catechol-O-Methyltransferase genes and their association with smoking risk in young men

Authors: Wei-Chih Ou, Yi-Chin Huang, Chih-Ling Huang, Min-Hsuan Lin, Yi-Chun Chen, Yi-Ju Chen, Chen-Nu Liu, Mei-Chih Chen, Ching-Shan Huang, Pei-Lain Chen

Published in: Behavioral and Brain Functions | Issue 1/2017

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Abstract

Background

Although some effects of gene–gene interactions on nicotine–dopamine metabolism for smoking behavior have been reported, polymorphisms of cytochrome P450 (CYP) 2A6 and catechol-O-methyltransferase (COMT) have not been studied together to determine their effects on smokers. The aim of this study was to investigate the effects of the interaction between the CYP 2A6 and COMT genes on smoking behavior in young Taiwanese men.

Results

A self-report questionnaire regarding smoking status was administered to 500 young men. Polymorphisms of the CYP 2A6 and COMT genes as well as urinary nicotine and urinary cotinine levels were determined. The odds ratio for starting smoking was significantly lower in subjects carrying a CYP2A6 low activity/variant COMT rs4680 genotype than in those possessing a CYP2A6 wild-type/variant COMT rs4680 genotype (0.44, 95% confidence interval = 0.19–0.98, P = 0.043). Comparisons of Fagerstrom Test for Nicotine Dependence (FTND), Physiological Cigarette Dependence Scale (PCDS), and Cigarette Withdrawal symptoms (CWS-21) among the smokers with different CYP2A6/COMT polymorphisms were not significantly different. The adjusted urinary nicotine concentrations were not significantly different between the two groups carrying different genotypes. The adjusted urinary cotinine level was significantly different between the COMT rs4680 wild-type group and COMT rs4680 variant group [92.46 ng/μL vs. 118.24 ng/μL (median value), P = 0.041] and between the COMT rs4680 wild-type/COMT rs165599 variant group and COMT rs4680 variant/COMT rs165599 variant group (97.10 ng/μL vs. 122.18 ng/μL, P = 0.022).

Conclusions

These findings suggest that a single nucleotide polymorphism (rs4680) of the COMT gene and the interaction between the CYP 2A6 and COMT genes affect smoking status in young Taiwanese men.
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Metadata
Title
Interaction between cytochrome P450 2A6 and Catechol-O-Methyltransferase genes and their association with smoking risk in young men
Authors
Wei-Chih Ou
Yi-Chin Huang
Chih-Ling Huang
Min-Hsuan Lin
Yi-Chun Chen
Yi-Ju Chen
Chen-Nu Liu
Mei-Chih Chen
Ching-Shan Huang
Pei-Lain Chen
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Behavioral and Brain Functions / Issue 1/2017
Electronic ISSN: 1744-9081
DOI
https://doi.org/10.1186/s12993-017-0127-2

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