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International Urogynecology Journal

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Open Access 15-03-2024 | Original Article

Effect of Vaginal Microecological Alterations on Female Pelvic Organ Prolapse

Authors: Shaozhan Chen, Qiaomei Zheng, Limin Zhang, Lihong Chen, Jinhua Wang

Published in: International Urogynecology Journal | Issue 4/2024

Abstract

Introduction and Hypothesis

The objective was to investigate the correlation between endogenous vaginal microecological alterations and female pelvic organ prolapse (POP).

Methods

Patients who underwent vaginal hysterectomy were retrospectively analyzed as the POP group (n = 30) and the non-POP group (n = 30). The vaginal microbial metabolites and enzyme levels were tested using the dry chemoenzymatic method. The mRNA and protein expression were tested using real-time quantitative PCR and immunohistochemistry. SPSS version 25.0 and GraphPad Prism 8.0 were performed for statistical analysis.

Results

Compared with the non-POP group, the vaginal pH, H₂O₂ positivity and leukocyte esterase positivity were higher in patients with POP (all p < 0.05). Further analysis showed that patients with pelvic organ prolapse quantification (POP-Q) stage IV had higher rates of vaginal pH, H₂O₂ positivity and leukocyte esterase positivity than those with POP-Q stage III. Additionally, the mRNA expression of decorin (DCN), transforming growth factor beta 1 (TGF-β1), and matrix metalloproteinase-3 (MMP-3) in uterosacral ligament tissues were higher, whereas collagen I and III were lower. Similarly, the positive expression of MMP-3 in uterosacral ligament tissue was significantly upregulated in the POP group compared with the non-POP group (p = 0.035), whereas collagen I (p = 0.004) and collagen III (p = 0.019) in uterosacral ligament tissue were significantly downregulated in the POP group. Correlation analysis revealed that there was a significant correlation between vaginal microecology and collagen metabolism. In addition, MMP-3 correlated negatively with collagen I and collagen III (p = 0.002, r = −0.533; p = 0.002, r = −0.534 respectively), whereas collagen I correlated positively with collagen III (p = 0.001, r = 0.578).

Conclusions

Vaginal microecological dysbiosis affects the occurrence of female POP, which could be considered a novel therapeutic option.

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Title: Effect of Vaginal Microecological Alterations on Female Pelvic Organ Prolapse
Authors: Shaozhan Chen
Qiaomei Zheng
Limin Zhang
Lihong Chen
Jinhua Wang
Publication date: 15-03-2024
Publisher: Springer International Publishing
Published in: International Urogynecology Journal / Issue 4/2024
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-024-05759-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of Vaginal Microecological Alterations on Female Pelvic Organ Prolapse

Abstract

Introduction and Hypothesis

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction and Hypothesis

Methods

Results

Conclusions

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