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Clinical Oral Investigations

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01-07-2020 | Original Article

Effect of static compressive force on in vitro cultured PDL fibroblasts: monitoring of viability and gene expression over 6 days

Authors: Mila Janjic Rankovic, Denitsa Docheva, Andrea Wichelhaus, Uwe Baumert

Published in: Clinical Oral Investigations | Issue 7/2020

Abstract

Objectives

The aim was to investigate the impact of static compressive force (CF) application on human PDL-derived fibroblasts (HPDF) in vitro for up to 6 days on the expression of specific genes and to monitor cell growth and cell viability.

Materials and methods

CF of 2 g/cm² was applied on HPDFs for 1–6 days. On each day, gene expression (cFOS, HB-GAM, COX2, IL6, TNFα, RUNX2, and P2RX2) and secretion (TNFα, PGE₂) were determined by RT-qPCR and ELISA, respectively. Cell growth and cell viability were monitored daily.

Results

In comparison with controls, significant upregulation of cFOS in compressed HPDFs was observed. HB-GAM showed no changes in expression, except on day 5 (P < 0.001). IL6 expression was significantly upregulated from day 2–5, reaching the maximum on day 3 (P < 0.001). TNFα expression was upregulated on all but day 2. COX2 showed upregulation, reaching the plateau from day 3 (P < 0.001) until day 4 (P < 0.001), and returning to the initial state till day 6. P2RX7 was downregulated on days 2 and 4 to 6 (P < 0.001). RUNX2 was downregulated on days 2 and 5 (both P < 0.001). Cells in both groups were proliferating, and no negative effect on cell viability was observed.

Conclusion

Results suggest high molecular activity up to 6 days, therefore introducing further need for in vitro studies with a longer duration that would explain other genes and metabolites involved in orthodontic tooth movement (OTM).

Clinical relevance

Extension of an established in vitro force application system for prolonged force application (6 days) simulating the initial phase of OTM.

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Title: Effect of static compressive force on in vitro cultured PDL fibroblasts: monitoring of viability and gene expression over 6 days
Authors: Mila Janjic Rankovic
Denitsa Docheva
Andrea Wichelhaus
Uwe Baumert
Publication date: 01-07-2020
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 7/2020
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-019-03113-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of static compressive force on in vitro cultured PDL fibroblasts: monitoring of viability and gene expression over 6 days

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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