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Clinical Oral Investigations

Published in:

01-09-2016 | Original Article

Effect of gaseous ozone on Enterococcus faecalis biofilm–an in vitro study

Authors: Tanja Boch, Christian Tennert, Kirstin Vach, Ali Al-Ahmad, Elmar Hellwig, Olga Polydorou

Published in: Clinical Oral Investigations | Issue 7/2016

Abstract

Objectives

The aim of this study was to evaluate the antimicrobial effect of gaseous ozone compared to conventional methods against Enterococcus faecalis.

Materials and methods

One hundred twenty-five teeth were infected by E. faecalis and were incubated for 72 h to form biofilm. Teeth were distributed among five groups. In the first group, ozone was used; in the second group, teeth were rinsed with 20 % ethylenediaminetetraacetic acid (EDTA); in the third group, with 3 % sodium hypochlorite (NaOCl). Group 4 combined 20 % EDTA with ozone. NaOCl and ozone were combined in group 5. After treatment, the samples with paper points were taken, followed by dentin samples taken with K-file, and cultured for 24 h. Then bacterial colonies were counted.

Results

All treatments reduced significantly (p < 0.05) the bacteria. Paper points’ samples showed 85.38 % reduction after ozone. The highest reduction was observed in NaOCl group (99.98 %). EDTA reduced bacteria by 80.64 %. Combination of NaOCl and ozone eradicated 99.95 % of the bacteria. Combination of EDTA and ozone reduced E. faecalis up to 91.33 %. The dentin chips showed the following: the highest CFU counts were observed in EDTA group, followed by ozone and NaOCl group. The lowest CFU counts were found in NaOCl-ozone group and EDTA-ozone group.

Conclusions

Ozone reduced E. faecalis, even organised in a biofilm, however, lower than NaOCl. No treatment reduced totally the bacteria.

Clinical relevance

Used as an adjuvant, ozone can increase the efficacy of conventional rinsing like EDTA and presents an alternative treatment when NaOCl cannot be used e.g. in teeth with a wide-open apical foramen.

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Title: Effect of gaseous ozone on Enterococcus faecalis biofilm–an in vitro study
Authors: Tanja Boch
Christian Tennert
Kirstin Vach
Ali Al-Ahmad
Elmar Hellwig
Olga Polydorou
Publication date: 01-09-2016
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 7/2016
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-015-1667-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of gaseous ozone on Enterococcus faecalis biofilm–an in vitro study

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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