Published in:
01-09-2007
Effect of 5-HT2 Receptor Blockade on Cadmium-Induced Acute Toxicity
Authors:
Konstantinos N. Tzirogiannis, Maria D. Demonakou, George K. Papadimas, Spyridon D. Skaltsas, Georgia A. Manta, Kalliopi T. Kourentzi, Katerina N. Alexandropoulou, Rosa I. Hereti, Michael G. Mykoniatis, Georgios I. Panoutsopoulos
Published in:
Digestive Diseases and Sciences
|
Issue 9/2007
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Abstract
The protective effect of 5-HT2 receptor blockade with ketanserin or ritanserin against cadmium liver injury was investigated. Male Wistar rats were injected intraperitoneally with a sublethal dose of cadmium (3.5 mg/kg body weight). Rats were treated with normal saline (group I), ketanserin (3 mg/kg body weight; group II), or ritanserin (3 mg/kg body weight; group III) 2 hr prior and 4 hr after cadmium administration and killed at different time points. Hematoxylin/eosin-stained liver sections were assessed for necrosis, apoptosis, peliosis, mitoses, and inflammatory infiltration. Apoptosis was also quantified by the TUNEL assay. Nonparenchymal liver cells and activated Kupffer cells were identified histochemically. Necrosis, hepatocyte apoptosis, nonparenchymal cell apoptosis, and macroscopic and microscopic peliosis were markedly reduced or minimized in ketanserin- or ritanserin-treated rats. The observed protective effect was almost identical for both ketanserin and ritanserin administration. 5-HT2 receptor blockade exerts a protective effect against acute cadmium-induced hepatotoxicity.