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01-09-2007 | Original Paper

Erdosteine Prevents Colonic Inflammation Through Its Antioxidant and Free Radical Scavenging Activities

Authors: Göksel Şener, Halil Aksoy, Özer Şehirli, Meral Yüksel, Cenk Aral, Nursal Gedik, Şule Çetinel, Berrak Ç. Yeğen

Published in: Digestive Diseases and Sciences | Issue 9/2007

Abstract

After intracolonic administration of trinitrobenzene sulphonic acid (TNBS), Sprague-Dawley rats were treated orally either with saline or erdosteine (100 mg/kg per day), a sulfhydryl-containing antioxidant, for 3 days. On the 4th day, rats were decapitated and distal colon was removed for the macroscopic and microscopic damage scoring, for the measurement of malondialdehyde (MDA), glutathione (GSH) and collagen levels, myeloperoxidase (MPO) activity, luminol and lucigenin chemiluminescences (CL) and DNA fragmentation. Lactate dehydrogenase (LDH) activity, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and antioxidant capacity were assayed in blood samples. Colitis caused significant increases in the colonic CL values, macroscopic and microscopic damage scores, MDA and collagen levels, MPO activity and DNA fragmentation, along with a significant decrease in tissue GSH level. Similarly, serum cytokines and LDH were elevated in the saline-treated colitis group as compared with the control group. On the other hand, erdosteine treatment reversed all these biochemical indices, and histopathologic alterations induced by TNBS, suggesting that erdosteine protects the colonic tissue via its radical scavenging and antioxidant activities.

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Title: Erdosteine Prevents Colonic Inflammation Through Its Antioxidant and Free Radical Scavenging Activities
Authors: Göksel Şener
Halil Aksoy
Özer Şehirli
Meral Yüksel
Cenk Aral
Nursal Gedik
Şule Çetinel
Berrak Ç. Yeğen
Publication date: 01-09-2007
Publisher: Springer US
Published in: Digestive Diseases and Sciences / Issue 9/2007
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-007-9801-9

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