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Open Access 01-12-2015 | Review

Dried blood spots PCR assays to screen congenital cytomegalovirus infection: a meta-analysis

Authors: Li Wang, Xiaoxing Xu, Huiping Zhang, Jihong Qian, Jianxing Zhu

Published in: Virology Journal | Issue 1/2015

Abstract

The performance of dried blood spots (DBS) polymerase chain reaction (PCR) assays in screening for congenital cytomegalovirus (cCMV) infection varies between different studies. To determine whether the DBS PCR assay has sufficient accuracy to be used as a screening test for cCMV infection, we performed a meta-analysis of 15 studies (n = 26007 neonates) that evaluated the performance of DBS PCR tests in screening for cCMV infection and that met our inclusion criteria. The pooled sensitivity and specificity were 0.844 (95% CI = 0.812–0.872) and 0.999 (95% CI = 0.998–0.999), respectively, and the diagnostic odds ratio was 1362.10 (95%CI = 566.91–3272.60). As sensitivity analysis showed that the results were robust. In conclusion, the performance of DBS PCR assays for testing cCMV was more suitable for retrospective diagnosis than screening.

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Title: Dried blood spots PCR assays to screen congenital cytomegalovirus infection: a meta-analysis
Authors: Li Wang
Xiaoxing Xu
Huiping Zhang
Jihong Qian
Jianxing Zhu
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2015
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-015-0281-9

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