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Open Access 01-12-2021 | Doxycycline | Research article

Combination effect of laser diode for photodynamic therapy with doxycycline on a wistar rat model of periodontitis

Authors: Suryani Dyah Astuti, Irawan Budi Utomo, Ernie Maduratna Setiawatie, Miratul Khasanah, Hery Purnobasuki, Deny Arifianto, Kartika Anggraini Alamsyah

Published in: BMC Oral Health | Issue 1/2021

Abstract

Background

Periodontitis is a chronic inflammatory disease characterized by progressive damage on the structure of tooth-supporting tissues. The aim of the study is determining the combination photodynamic effect of diode laser 405 nm treatments and the administration of doxycycline 0.1% within 1, 3, 5, and 7 days on a Wistar rat model of periodontitis.

Methods

Samples were induced with Porphyromonas gingivalis ATCC33277 to allow periodontitis development and were treated with combination of doxycycline and laser diode, then statistical analysis was carried out (One-Way ANOVA test and the post-hoc Duncan test; Kruskal–Wallis test and Mann–Whitney follow-up test for non-parametric data). Samples were divided into five groups, laser exposure used was 405-nm diode laser with energy density of 8 J/cm². The expression level of histomorphometric was calculated by measuring the number of macrophages, lymphocytes, fibroblasts and the distance between the CEJ-AV.

Results

The results showed that the combination treatment of doxycycline and laser exposure yielded immunomodulatory effects. The expression level of fibroblast and the distance between CEJ-AV bone showed that the combination of doxycycline and laser therapy exerted healing effect in rat models of periodontitis on day 5 and 7.

Conclusion

The combination of doxycycline 0.1% and diode laser therapy provides a healing effect in rats models of periodontitis.

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Title: Combination effect of laser diode for photodynamic therapy with doxycycline on a wistar rat model of periodontitis
Authors: Suryani Dyah Astuti
Irawan Budi Utomo
Ernie Maduratna Setiawatie
Miratul Khasanah
Hery Purnobasuki
Deny Arifianto
Kartika Anggraini Alamsyah
Publication date: 01-12-2021
Publisher: BioMed Central
Keywords: Doxycycline
Photodynamic Therapy
Diode Laser
Laser
Published in: BMC Oral Health / Issue 1/2021
Electronic ISSN: 1472-6831
DOI: https://doi.org/10.1186/s12903-021-01435-0

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Combination effect of laser diode for photodynamic therapy with doxycycline on a wistar rat model of periodontitis

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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