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Sleep and Breathing
Published in: Sleep and Breathing 3/2014

01-09-2014 | Original Article

Downregulation of uncoupling protein-1 mRNA expression and hypoadiponectinemia in a mouse model of sleep apnea

Authors: Cintia Zappe Fiori, Denis Martinez, Diego Baronio, Darlan Pase da Rosa, Nelson Alexandre Kretzmann, Luiz Felipe Forgiarini, Carolina Caruccio Montanari, Norma Possa Marroni, Alicia Carissimi

Published in: Sleep and Breathing | Issue 3/2014

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Abstract

Purpose

The knowledge on the effect of intermittent hypoxia on adipose tissue-mediated processes is incipient. The aim of the present study was to assess the effect of a sleep apnea model on a limited set of specific molecular, biochemical, histological, and behavioral parameters of adipose tissue function.

Methods

Mice were exposed to either intermittent hypoxia or sham hypoxia during 8 h a day for 37 days. Uncoupling protein-1 expression in brown adipose tissue was measured by real-time PCR and immunohistochemistry. Digital quantification of adipose cells and immunohistochemistry of uncoupling protein-1 were performed to determine cell dimensions, positive area, and staining intensity. Serum levels of leptin, adiponectin, and cortisol were measured by ELISA.

Results

In comparison with the control group, animals in the hypoxia group had significantly lower chow ingestion, weight gain, and smaller white and brown adipocytes on histological examination. Adiponectin levels were also lower in the hypoxia group. Uncoupling protein-1 mRNA was abolished in the mice exposed to hypoxia; accordingly, fewer cells positive for uncoupling protein-1 and lighter staining intensity were observed in brown adipocytes.

Conclusions

An experimental model of sleep apnea produced changes in uncoupling protein-1 expression and adiponectin levels. These results confirm previous findings on the response of brown adipose tissue to intermittent hypoxia and indicate a yet-unknown interference of intermittent hypoxia on energy control, which may participate in the propensity to weight gain observed in patients with sleep apnea. Brown adipose tissue activity in this patient population needs to be further investigated.
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Metadata
Title
Downregulation of uncoupling protein-1 mRNA expression and hypoadiponectinemia in a mouse model of sleep apnea
Authors
Cintia Zappe Fiori
Denis Martinez
Diego Baronio
Darlan Pase da Rosa
Nelson Alexandre Kretzmann
Luiz Felipe Forgiarini
Carolina Caruccio Montanari
Norma Possa Marroni
Alicia Carissimi
Publication date
01-09-2014
Publisher
Springer Berlin Heidelberg
Published in
Sleep and Breathing / Issue 3/2014
Print ISSN: 1520-9512
Electronic ISSN: 1522-1709
DOI
https://doi.org/10.1007/s11325-013-0916-2

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