Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Clinical Oral Investigations
Published in: Clinical Oral Investigations 1/2020

01-01-2020 | Digital Volume Tomography | Original Article

Imaging of nano-hydroxyapatite/chitosan scaffolds using a cone beam computed tomography device on rat calvarial defects with histological verification

Authors: Emmanouil Chatzipetros, Zafeiroula Yfanti, Panos Christopoulos, Catherine Donta, Spyros Damaskos, Evangelos Tsiambas, Dimitris Tsiourvas, Eleni-Marina Kalogirou, Konstantinos I. Tosios, Kostas Tsiklakis

Published in: Clinical Oral Investigations | Issue 1/2020

Login to get access

Abstract

Objectives

Τhis study aims at determining the ability of cone beam computed tomography (CBCT) to visualize critical-size defects (CSD) created at rat calvaria and filled with 75/25 w/w nano-hydroxyapatite/chitosan (nHAp/CS) scaffolds, prior to their histological investigation.

Materials and methods

Thirty adult Sprague Dawley rats, 15 males and 15 females, were used. Two CSD, 5 mm in diameter, were bilaterally trephined in the parietal bone. The right CSD was filled with nHAp/CS scaffold, while the left CSD remained empty, as the control group. Two female rats died post-operatively. Rats were euthanized at 2, 4, and 8 weeks post-surgery. Twenty-eight specimens (15 × 2 × 10 mm) were resected—containing both CSDs—and then scanned using a NewTom VGi CBCT imaging unit (Verona, Italy). The manufacturer’s software trace region profile tool (NNT v6.2, Verona, Italy) was used in selected axial slices. The greyscale value (in VGiHU) and the traced/selected region of interest (ROI, in mm2) of those areas were automatically calculated. Subsequently, all specimens were histologically examined.

Results

An increased VGiHU (P = 0.000), was observed in the experimental group relative to the control group. The ROI of CSD (in mm2) was significantly reduced (P = 0.001) from the fourth to the eighth week in both groups. No statistically significant difference between male and female rats (P = 0.188) was observed with respect to VGiHU.

Conclusions

The nHAp/CS scaffolds are easily visualized using a particular high-resolution CBCT device.

Clinical relevance

Both the CBCT measurements and also the histological results suggest that the nHAp/CS scaffold presence contributes to new bone formation in rat calvarial CSD.
Literature
1.
Gomes PS, Fernandes MH (2011) Rodent models in bone-related research: the relevance of calvarial defects in the assessment of bone regeneration strategies. Lab Anim 45:14–24PubMedCrossRef
2.
Bosch C, Melsen B, Vargervik K (1998) Importance of the critical-size bone defect in testing bone-regenerating materials. J Craniofac Surg 9:310–316PubMedCrossRef
3.
Donos N, Lang NP, Karoussis IK, Bosshardt D, Tonetti M, Kostopoulos L (2004) Effect of GBR in combination with deproteinized bovine bone mineral and/or enamel matrix proteins on the healing of critical-size defects. Clin Oral Implants Res 15:101–111PubMedCrossRef
4.
Donos N, Dereka X, Mardas N (2015) Experimental models for guided bone regeneration in healthy and medically compromised conditions. Periodontol 68:99–121CrossRef
5.
Ghiacci G, Graiani G, Ravanetti F, Lumetti S, Manfredi E, Galli C, Cacchioli A, Macaluso GM, Sala R (2016) “Over-inlay” block graft and differential morphometry: a novel block graft model to study bone regeneration and host-to-graft interfaces in rats. J Periodontal Implant Sci 46(4):220–223PubMedPubMedCentralCrossRef
6.
Pryor ME, Polimeni G, Koo KT, Hartman MJ, Gross H, April M et al (2005) Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: histologic and histometric observations. J ClinPeriodontol 32:966–972
7.
Buser D, Hoffman B, Bernard JP, Lussi A, Mettler D, Schenk RK (1998) Evaluation of filling materials in membrane protected bone defects. A comparative histomorphometric study in the mandible of miniature pigs. Clin Oral Implants Res 9:137–150PubMedCrossRef
8.
9.
Kurz LT, Garfin SR, Booth REJ (1989) Harvesting autogenous iliac bone grafts: a review of complications and techniques. Spine 14:1324–1331PubMedCrossRef
10.
Shalash MA, Rahman HA, Azim AA, Neemat AH, Hawary HE, Nasry SA (2013) Evaluation of horizontal ridge augmentation using beta tricalcium phosphate and demineralized bone matrix: a comparative study. J Clin Exp Dent 5:253–259CrossRef
11.
12.
13.
14.
Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR (2006) Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials 27:3413–3431PubMedCrossRef
15.
Bonucci E (2000) Basic composition and structure of bone. In: An YH, Draughn RA (eds) Mechanical testing of bone and the bone-implant interface. CRC Press LLC Boca Raton, USA, pp 3–21
16.
Tsiourvas D, Sapalidis A, Papadopoulos T (2016) Hydroxyapatite/chitosan-based porous three-dimensional scaffolds with complex geometries. Mater Today 7:59–66
17.
O’Brien FJ (2011) Biomaterials and scaffolds for tissue engineering. Mater Today 14:88–95CrossRef
18.
Vlierberghe SV, Dubruel P, Schacht E (2011) Biopolymer-based hydrogels as Scaffolds for tissue engineering applications: a review. Biomacromolecules 2:1387–1408CrossRef
19.
Kong L, Gao Y, Lu G, Gong Y, Zhao N, Zhang X (2006) A study on the bioactivity of chitosan/nano-hydroxyapatite composite scaffolds for bone tissue engineering. Eur Polym J 42:3171–3179CrossRef
20.
Kashiwazaki H, Kishiya Y, Matsuda A, Yamaguchi K, Iizuka T, Tanaka J, Inoue N (2009) Fabrication of porous chitosan/hydroxyapatite nanocomposites: their mechanical and biological properties. BioMed Mater Eng 19:133–140PubMed
21.
Thein-Han WW, Misra RDK (2009) Three-dimensional chitosan-nanohydroxyapatite composite scaffolds for bone tissue engineering. JOM J Miner Met Mater Soc 61:41–44CrossRef
22.
23.
Blaker JJ, Gough JE, Maquet V, Notingher I, Boccaccini AR (2003) In vitro evaluation of novel bioactive composites based on bioglass-filledpolylactide foams for bone tissue engineering scaffolds. J Biomed Mater Res 67A:1401–1411CrossRef
24.
Castro F, Kuhn S, Jensen K, Ferreira A, Rocha F, Vicente A et al (2013) Continuous-flow precipitation of hydroxyapatite in ultrasonic microsystems. Chem Eng J 215:979–987CrossRef
25.
Cui W, Li X, Xie C, Chen J, Zou J, Zhou S, Weng J (2010) Controllable growth of hydroxyapatite on electrospun poly(dl-lactide) fibers grafted with chitosan as potential tissue engineering scaffolds. Polymer 51:2320–2328CrossRef
26.
Di Martino A, Sittinger M, Risbud MV (2005) Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. Biomaterials 26:5983–5990PubMedCrossRef
27.
Tigli RS, Karakecili A, Gumusderelioglu M (2007) In vitro characterization of chitosan scaffolds: influence of composition and deacetylation degree. J Mater Sci Mater Med 18:1665–1674CrossRef
28.
Nakamura T, Shirakata Y, Shinohara Y, Miron RJ, Hasegawa-Nakamura K, Fujioka-Kobayashi M, Noguchi K (2017) Comparison of the effects of recombinant human bone morphogenetic protein-2 and -9 on bone formation in rat calvarial critical-size defects. Clin Oral Investig 21(9):2671–2679PubMedCrossRef
29.
Zhang H, Mao X, Du Z, Jiang W, Han X, Zhao D et al (2016) Three dimensional printed macroporouspolylactic acid/hydroxyapatite composite scaffolds for promoting bone formation in a critical-size rat calvarial defect model. Sci Technol Adv Mater 17:136–148PubMedPubMedCentralCrossRef
30.
Ito K, Gomi Y, Sato S, Arai Y, Shinoda K (2001) Clinical application of a new compact CT system to assess 3-D images for the preoperative treatment planning of implants in the posterior mandible. A case report. Clin Oral Implants Res 12:539–542PubMedCrossRef
31.
Pauwels R, Beinsberger J, Collaert B, Theodorakou C, Rogers J, Walker A, Cockmartin L, Bosmans H, Jacobs R, Bogaerts R, Horner K, SEDENTEXCT Project Consortium (2012) Effective dose range for dental cone beam computed tomography scanners. Eur J Radiol 81:267–271PubMedCrossRef
32.
33.
Lagravère MO, Carey J, Toogood RW, Major PW (2008) Three-dimensional accuracy of measurements made with software on cone-beam computed tomography images. Am J Orthod Dentofac Orthop 134:112–116CrossRef
34.
Aranyarachkul P, Caruso J, Gantes B, Schulz E, Riggs M, Dus I, Yamada JM, Crigger M (2005) Bone density assessments of dental implant sites. 2. Quantitative cone-beam computerized tomography. Int J Oral Maxillofac Implants 20:416–424PubMed
35.
Naitoh M, Hirukawa A, Katsumata A, Ariji E (2009) Evaluation of voxel values in mandibular cancellous bone: relationship between cone-beam computed tomography and multislice helical computed tomography. Clin Oral Implants Res 20:503–506PubMedCrossRef
36.
Naitoh M, Hirukawa A, Katsumata A, Ariji E (2010) Prospective study to estimate mandibular cancellous bone density using large-volume cone-beam computed tomography. Clin Oral Implants Res 21:1309–1313PubMedCrossRef
37.
Nomura Y, Watanabe H, Honda E, Kurabayashi T (2010) Reliability of voxel values from cone-beam computed tomography for dental use in evaluating bone mineral density. Clin Oral Implants Res 21:558–562PubMedCrossRef
38.
Lagravère MO, Fang Y, Carey J, Toogood RW, Packota GV, Major PW (2006) Density conversion factor determined using a cone-beam computed tomography unit NewTom QR-DVT 9000. DentomaxillofacRadiol 35:407–409CrossRef
39.
Campos MJ, de Souza TS, MotaJúnior SL, Fraga MR, Vitral RW (2014) Bone mineral density in cone beam computed tomography: only a few shades of gray. World J Radiol 28;6(8):607–612PubMedPubMedCentralCrossRef
40.
41.
Ponder SN, Benavides E, Kapila S, Hatch NE (2013) Quantification of external root resorption by low-vs high- resolution cone-beam computed tomography and periapical radiography: a volumetric and linear analysis. Am J Orthod Dentofac Orthop 143:77–91CrossRef
42.
Van Dessel J, Nicolielo LFP, Huang Y, Coudyzer W, Salmon B, Lambrichts I, Jacobs R (2017) Accuracy and reliability of different cone beam computed tomography (CBCT) devices for structural analysis of alveolar bone in comparison with multislice CT and micro-CT. Eur J Oral Implantol 10(1):95–105PubMed
43.
Liang X, Zhang Z, Gu J, Wang Z, Vandenberghe B, Jacobs R, Yang J, Ma G, Ling H, Ma X (2017) Comparison of micro-CT and cone beam CT on the feasibility of assessing trabecular structures in mandibular condyle. Dentomaxillofac Radiol 46:20160435PubMedPubMedCentralCrossRef
44.
45.
Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG (2010) Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biol 8(6):e1000412PubMedPubMedCentralCrossRef
46.
Tsiourvas D, Tsetsekou A, Kammenou MI, Boukos N (2011) Controlling the formation of hydroxyapatite nanorods with dendrimers. J Am Ceram Soc 94:2023–2029CrossRef
47.
Kuo YC, Tsai YT (2010) Inverted colloidal crystal scaffolds for uniform cartilage regeneration. Biomacromolecules 11:731–739PubMedCrossRef
48.
Kim RW, Kim JH, Moon SY (2016) Effect of hydroxyapatite on critical-sized defect. Maxillofac Plast Reconstr Surg 38:1–6CrossRef
49.
Gao R, Watson M, Callon KE, Tuari D, Dray M, Naot D et al (2018) Local application of lactoferrin promotes bone regeneration in a rat critical-sized calvarial defect model as demonstrated by micro-CT and histological analysis. J Tissue Eng Regen Med 12:620–626CrossRef
50.
Johari B, Ahmadzadehzarajabad M, Azami M, Kazemi M, Soleimani M, Kargozar S, Hajighasemlou S, Farajollahi MM, Samadikuchaksaraei A (2016) Repair of rat critical size calvarial defect using osteoblast-like and umbilical vein endothelial cells seeded in gelatin/hydroxyapatite scaffolds. J Biomed Mater Res 104:1770–1778CrossRef
51.
Oryan A, Alidadi S, Bigham-Sadegh A, Meimandi-Parizi A (2017) Chitosan/gelatin/platelet gel enriched by o combination of hydroxyapatite and beta-tricalcium phosphate in healing of radial bone defect model in rat. Int J Biol Macromol 101:630–637PubMedCrossRef
52.
He Y, Dong Y, Cui F, Chen X, Lin R (2015) Ectopic osteogenesis and scaffold biodegradation of nano-hydroxyapatite-chitosan in a rat model. PLoS One 10:1–15
53.
Groppo MF, Caria PH, Freire AR, Figueroba SR, Ribeiro-Neto WA, Bretas RES (2017) The effect of a hydroxyapatite impregnated PCL membrane in rat subcritical calvarial bone defects. Arch Oral Biol 82:209–215PubMedCrossRef
54.
Lohmann P, Willuweit A, Neffe AT, Geisler S, Gebauer TP, Beer S, Coenen HH, Fischer H, Hermanns-Sachweh B, Lendlein A, Shah NJ, Kiessling F, Langen KJ (2017) Bone regeneration induced by a 3D architectured hydrogel in a rat critical-size calvarial defect. Biomaterials 113:158–169PubMedCrossRef
55.
de Santana WM, de Sousa DN, Ferreira VM, Duarte WR (2016) Simvastatin and biphasic calcium phosphate affects bone formation in critical-sized rat calvarial defects. Acta Cir Bras 31:300–307PubMedCrossRef
56.
57.
Pauwels R, Jacobs R, Singer SR, Mupparapu M (2015) CBCT-based bone quality assessment: are Hounsfield units applicable? DentomaxillofacRadiol 44:1–16
58.
Cankaya AB, Erdem MA, Isler SC, Demircan S, Soluk M, Kasapoglu C, Oral CK (2011) Use of cone-beam computerized tomography for evaluation of bisphosphonate-associated osteonecrosis of the jaws in an experimental rat model. Int J Med Sci 8(8):667–672PubMedPubMedCentralCrossRef
59.
Chatzipetros E, Christopoulos P, Donta C, Tosios KI, Tsiambas E, Tsiourvas D, Kalogirou EM, Tsiklakis K (2018) Application of nano-hydroxyapatite/chitosan scaffolds on rat calvarial critical-sized defects: a pilot study. Med Oral Patol Oral Cir Bucal 23(5):625–632
Metadata
Title
Imaging of nano-hydroxyapatite/chitosan scaffolds using a cone beam computed tomography device on rat calvarial defects with histological verification
Authors
Emmanouil Chatzipetros
Zafeiroula Yfanti
Panos Christopoulos
Catherine Donta
Spyros Damaskos
Evangelos Tsiambas
Dimitris Tsiourvas
Eleni-Marina Kalogirou
Konstantinos I. Tosios
Kostas Tsiklakis
Publication date
01-01-2020
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 1/2020
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-019-02939-4

Other articles of this Issue 1/2020

Clinical Oral Investigations 1/2020 Go to the issue

Original Article

Duration of ultrasonic activation causing secondary fractures during the removal of the separated instruments with different tapers

Original Article

The outcome of a temporomandibular joint compression test for the diagnosis of arthralgia is confounded by concurrent myalgia

Original Article

Reciproc and Reciproc Blue in the removal of bioceramic and resin-based sealers in retreatment procedures

Original Article

Variation of human salivary alpha-amylase proteoforms in three stimulation models

Review

The assessment of stress, depression, and inflammation as a collective risk factor for periodontal diseases: a systematic review

Original Article

Oral health–related quality of life in different clinical forms of oral lichen planus