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European Journal of Nutrition
Published in: European Journal of Nutrition 3/2017

01-04-2017 | Original Contribution

Dietary and lifestyle determinants of acrylamide and glycidamide hemoglobin adducts in non-smoking postmenopausal women from the EPIC cohort

Authors: Mireia Obón-Santacana, Leila Lujan-Barroso, Heinz Freisling, Claire Cadeau, Guy Fagherazzi, Marie-Christine Boutron-Ruault, Rudolf Kaaks, Renée T. Fortner, Heiner Boeing, J. Ramón Quirós, Esther Molina-Montes, Saioa Chamosa, José María Huerta Castaño, Eva Ardanaz, Kay-Tee Khaw, Nick Wareham, Tim Key, Antonia Trichopoulou, Pagona Lagiou, Androniki Naska, Domenico Palli, Sara Grioni, Rosario Tumino, Paolo Vineis, Maria Santucci De Magistris, H. B. Bueno-de-Mesquita, Petra H. Peeters, Maria Wennberg, Ingvar A. Bergdahl, Hubert Vesper, Elio Riboli, Eric J. Duell

Published in: European Journal of Nutrition | Issue 3/2017

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Abstract

Purpose

Acrylamide was classified as ‘probably carcinogenic’ to humans in 1994 by the International Agency for Research on Cancer. In 2002, public health concern increased when acrylamide was identified in starchy, plant-based foods, processed at high temperatures. The purpose of this study was to identify which food groups and lifestyle variables were determinants of hemoglobin adduct concentrations of acrylamide (HbAA) and glycidamide (HbGA) in 801 non-smoking postmenopausal women from eight countries in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort.

Methods

Biomarkers of internal exposure were measured in red blood cells (collected at baseline) by high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) . In this cross-sectional analysis, four dependent variables were evaluated: HbAA, HbGA, sum of total adducts (HbAA + HbGA), and their ratio (HbGA/HbAA). Simple and multiple regression analyses were used to identify determinants of the four outcome variables. All dependent variables (except HbGA/HbAA) and all independent variables were log-transformed (log2) to improve normality. Median (25th–75th percentile) HbAA and HbGA adduct levels were 41.3 (32.8–53.1) pmol/g Hb and 34.2 (25.4–46.9) pmol/g Hb, respectively.

Results

The main food group determinants of HbAA, HbGA, and HbAA + HbGA were biscuits, crackers, and dry cakes. Alcohol intake and body mass index were identified as the principal determinants of HbGA/HbAA. The total percent variation in HbAA, HbGA, HbAA + HbGA, and HbGA/HbAA explained in this study was 30, 26, 29, and 13 %, respectively.

Conclusions

Dietary and lifestyle factors explain a moderate proportion of acrylamide adduct variation in non-smoking postmenopausal women from the EPIC cohort.
Appendix
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Literature
1.
Friedman M (2003) Chemistry, biochemistry, and safety of acrylamide. A review. J Agric Food Chem 51:4504–4526CrossRef
2.
Tareke E, Rydberg P, Karlsson P et al (2002) Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J Agric Food Chem 50:4998–5006CrossRef
3.
Becalski A, Brady B, Feng S et al (2011) Formation of acrylamide at temperatures lower than 100 °C: the case of prunes and a model study. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 28:726–730. doi:10.​1080/​19440049.​2010.​535217 CrossRef
4.
5.
Freisling H, Moskal A, Ferrari P et al (2013) Dietary acrylamide intake of adults in the European Prospective Investigation into Cancer and Nutrition differs greatly according to geographical region. Eur J Nutr 52:1369–1380CrossRef
6.
World Health Organization (2011) Evaluation of certain food additives and contaminants: seventy-second report of the joint FAO/WHO expert committee on food additives. World Health Organization Technical Report Series, No. 960, pp 1–115
7.
8.
LoPachin RM, Gavin T (2008) Acrylamide-induced nerve terminal damage: relevance to neurotoxic and neurodegenerative mechanisms. J Agric Food Chem 56:5994–6003CrossRef
9.
IARC (1994) IARC working group on the evaluation of carcinogenic risks to humans: some industrial chemicals. Lyon, 15–22 February 1994. IARC Monogr Eval Carcinog Risks Hum 60:1–560
10.
Fennell TR, Sumner SC, Walker VE (1992) A model for the formation and removal of hemoglobin adducts. Cancer Epidemiol Biomark Prev 1:213–219
11.
Bergmark E (1997) Hemoglobin adducts of acrylamide and acrylonitrile in laboratory workers, smokers and nonsmokers. Chem Res Toxicol 10:78–84CrossRef
12.
Vesper HW, Bernert JT, Ospina M et al (2007) Assessment of the relation between biomarkers for smoking and biomarkers for acrylamide exposure in humans. Cancer Epidemiol Biomark Prev 16:2471–2478CrossRef
13.
14.
Vesper HW, Slimani N, Hallmans G et al (2008) Cross-sectional study on acrylamide hemoglobin adducts in subpopulations from the European Prospective Investigation into Cancer and Nutrition (EPIC) Study. J Agric Food Chem 56:6046–6053CrossRef
15.
Ferrari P, Freisling H, Duell EJ et al (2013) Challenges in estimating the validity of dietary acrylamide measurements. Eur J Nutr 52:1503–1512CrossRef
16.
Bjellaas T, Olesen PT, Frandsen H et al (2007) Comparison of estimated dietary intake of acrylamide with hemoglobin adducts of acrylamide and glycidamide. Toxicol Sci 98:110–117CrossRef
17.
18.
Wilson KM, Vesper HW, Tocco P et al (2009) Validation of a food frequency questionnaire measurement of dietary acrylamide intake using hemoglobin adducts of acrylamide and glycidamide. Cancer Causes Control 20:269–278CrossRef
19.
Riboli E, Hunt KJ, Slimani N et al (2002) European Prospective Investigation into Cancer and Nutrition (EPIC): study populations and data collection. Public Health Nutr 5:1113–1124CrossRef
20.
Obón-Santacana M, Freisling H, Peeters PH et al (2015) Acrylamide and glycidamide hemoglobin adduct levels and endometrial cancer risk: a nested case-control study in nonsmoking postmenopausal women from the EPIC cohort. Int J Cancer. doi:10.​1002/​ijc.​29853
21.
Obón-Santacana M, Lujan-Barroso L, Travis RC et al (2015) Acrylamide and glycidamide hemoglobin adducts and epithelial ovarian cancer: a nested case-control study in non-smoking postmenopausal women from the EPIC cohort. Cancer Epidemiol Biomark Prev. doi:10.​1158/​1055-9965.​EPI-15-0822
22.
Cust AE, Kaaks R, Friedenreich C et al (2007) Metabolic syndrome, plasma lipid, lipoprotein and glucose levels, and endometrial cancer risk in the European Prospective Investigation into Cancer and Nutrition (EPIC). Endocr Relat Cancer 14:755–767. doi:10.​1677/​ERC-07-0132 CrossRef
23.
Peeters PH, Lukanova A, Allen N et al (2007) Serum IGF-I, its major binding protein (IGFBP-3) and epithelial ovarian cancer risk: the European Prospective Investigation into Cancer and Nutrition (EPIC). Endocr Relat Cancer 14:81–90CrossRef
24.
Obon-Santacana M, Slimani N, Lujan-Barroso L et al (2013) Dietary intake of acrylamide and pancreatic cancer risk in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort. Ann Oncol 24:2645–2651CrossRef
25.
Vesper HW, Ospina M, Meyers T et al (2006) Automated method for measuring globin adducts of acrylamide and glycidamide at optimized Edman reaction conditions. Rapid Commun Mass Spectrom 20:959–964CrossRef
26.
27.
Wilson KM, Balter K, Adami HO et al (2009) Acrylamide exposure measured by food frequency questionnaire and hemoglobin adduct levels and prostate cancer risk in the Cancer of the Prostate in Sweden Study. Int J Cancer 124:2384–2390CrossRef
28.
29.
Akaike H (1974) A new look at the statistical model identification. IEEE Trans Autom Control 19:716–723CrossRef
30.
Wareham NJ, Jakes RW, Rennie KL et al (2003) Validity and repeatability of a simple index derived from the short physical activity questionnaire used in the European Prospective Investigation into Cancer and Nutrition (EPIC) study. Public Health Nutr 6:407–413CrossRef
31.
McGraw KO, Wong SP (1996) Forming inferences about some intraclass correlation coefficients. Psychol Methods 1:30CrossRef
32.
33.
34.
Wirfalt E, Paulsson B, Tornqvist M et al (2008) Associations between estimated acrylamide intakes, and hemoglobin AA adducts in a sample from the Malmo Diet and Cancer cohort. Eur J Clin Nutr 62:314–323CrossRef
35.
36.
Vikstrom AC, Wilson KM, Paulsson B et al (2010) Alcohol influence on acrylamide to glycidamide metabolism assessed with hemoglobin-adducts and questionnaire data. Food Chem Toxicol 48:820–824CrossRef
37.
38.
Vesper HW, Sternberg MR, Frame T, Pfeiffer CM (2013) Among 10 sociodemographic and lifestyle variables, smoking is strongly associated with biomarkers of acrylamide exposure in a representative sample of the US population. J Nutr 143:995S–1000SCrossRef
39.
Kotova N, Frostne C, Abramsson-Zetterberg L et al (2014) Differences in micronucleus frequency and acrylamide adduct levels with hemoglobin between vegetarians and non-vegetarians. Eur J Nutr. doi:10.​1007/​s00394-014-0796-7
40.
Hogervorst JG, Baars BJ, Schouten LJ et al (2010) The carcinogenicity of dietary acrylamide intake: a comparative discussion of epidemiological and experimental animal research. Crit Rev Toxicol 40:485–512CrossRef
41.
Hogervorst JG, Fortner RT, Mucci LA et al (2013) Associations between dietary acrylamide intake and plasma sex hormone levels. Cancer Epidemiol Biomark Prev 22:2024–2036CrossRef
42.
Nagata C, Konishi K, Tamura T et al (2015) Associations of acrylamide intake with circulating levels of sex hormones and prolactin in premenopausal Japanese women. Cancer Epidemiol Biomark Prev 24:249–254. doi:10.​1158/​1055-9965.​EPI-14-0935 CrossRef
43.
Metadata
Title
Dietary and lifestyle determinants of acrylamide and glycidamide hemoglobin adducts in non-smoking postmenopausal women from the EPIC cohort
Authors
Mireia Obón-Santacana
Leila Lujan-Barroso
Heinz Freisling
Claire Cadeau
Guy Fagherazzi
Marie-Christine Boutron-Ruault
Rudolf Kaaks
Renée T. Fortner
Heiner Boeing
J. Ramón Quirós
Esther Molina-Montes
Saioa Chamosa
José María Huerta Castaño
Eva Ardanaz
Kay-Tee Khaw
Nick Wareham
Tim Key
Antonia Trichopoulou
Pagona Lagiou
Androniki Naska
Domenico Palli
Sara Grioni
Rosario Tumino
Paolo Vineis
Maria Santucci De Magistris
H. B. Bueno-de-Mesquita
Petra H. Peeters
Maria Wennberg
Ingvar A. Bergdahl
Hubert Vesper
Elio Riboli
Eric J. Duell
Publication date
01-04-2017
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Nutrition / Issue 3/2017
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-016-1165-5

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