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Archives of Virology
Published in: Archives of Virology 8/2016

01-08-2016 | Brief Report

Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil

Authors: Joana D. P. Mascarenhas, Delana A. M. Bezerra, René R. Silva, Mayara J. M. Silva, Edivaldo C. Sousa Júnior, Luana S. Soares

Published in: Archives of Virology | Issue 8/2016

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Abstract

The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens of broiler chickens in Brazil. During 2008 and 2011, a total of 85 fecal samples were collected. The viral genome was extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequencing. Samples were screened for rotaviruses by PAGE, and RVF and RVG genome banding patterns were not seen. Using RT-PCR, it was found that 9.4 % (8/85) of the pools contained RVF, whereas 10.6 % (9/85) contained RVG. The predicted amino acid sequences of RVF and RVG from Brazilian samples were 94.4-95.7 % and 96.8-96.9 % identical, respectively, to those of prototypes from Germany. The detection of RVF and RVG in this study provides important epidemiological data about the simultaneous circulation of rotaviruses affecting broiler flocks in the Amazon region of Brazil.
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Metadata
Title
Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil
Authors
Joana D. P. Mascarenhas
Delana A. M. Bezerra
René R. Silva
Mayara J. M. Silva
Edivaldo C. Sousa Júnior
Luana S. Soares
Publication date
01-08-2016
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-2882-z

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