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Open Access 01-12-2016 | Research

Detection of circulating tumor cells by p75NTR expression in patients with esophageal cancer

Authors: Tetsuji Yamaguchi, Tomoyuki Okumura, Katsuhisa Hirano, Toru Watanabe, Takuya Nagata, Yutaka Shimada, Kazuhiro Tsukada

Published in: World Journal of Surgical Oncology | Issue 1/2016

Abstract

Background

The p75 neurotrophin receptor (p75NTR) is a cancer stem cell (CSC) marker in esophageal squamous cell carcinoma (ESCC). This study aimed to assess the use of p75NTR in detecting circulating tumor cells (CTCs) in ESCC.

Methods

Peripheral blood mononuclear cell expression of epithelial cell adhesion molecule (EpCAM) and p75NTR was detected in 23 ESCC patients (13 received chemo- or chemoradiotherapy and 10 received curative surgery) and 10 healthy controls by flow cytometry.

Results

EpCAM + p75NTR+ cell counts (average ± SD) were significantly higher in patients (n = 23, 16.0 ± 18.3) compared to controls (n = 10, 0.4 ± 0.9, p = 0.013). The sensitivity and specificity to differentiate ESCC patients from controls were 78.3 and 100 % (cut-off value 4.0), respectively. EpCAM + p75NTR+, but not EpCAM + p75NTR− cell counts, correlated with clinically diagnosed distant metastasis (n = 13, p = 0.006) and pathological venous invasion in resected primary tumors (n = 10, p = 0.016). Malignant cytology was microscopically confirmed in isolated EpCAM + p75NTR+ cells with immunocytochemical double staining.

Conclusions

p75NTR is suggested to be a useful marker for clinically significant CTCs, which exhibit highly metastatic features in ESCC.

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Title: Detection of circulating tumor cells by p75NTR expression in patients with esophageal cancer
Authors: Tetsuji Yamaguchi
Tomoyuki Okumura
Katsuhisa Hirano
Toru Watanabe
Takuya Nagata
Yutaka Shimada
Kazuhiro Tsukada
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: World Journal of Surgical Oncology / Issue 1/2016
Electronic ISSN: 1477-7819
DOI: https://doi.org/10.1186/s12957-016-0793-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Detection of circulating tumor cells by p75NTR expression in patients with esophageal cancer

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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