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Virology Journal
Published in: Virology Journal 1/2023

Open Access 01-12-2023 | Dengue Virus | Brief Report

Genotyping of dengue virus from infected tissue samples embedded in paraffin

Authors: Jorge Alonso Rivera, Aura Caterine Rengifo, Alicia Rosales-Munar, Taylor H. Díaz-Herrera, José Usme Ciro, Edgar Parra, Diego A. Alvarez-Díaz, Katherine Laiton-Donato, María Leonor Caldas

Published in: Virology Journal | Issue 1/2023

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Abstract

Dengue has become one of the vector-borne diseases that affect humans worldwide. In Latin American countries, Colombia is historically one of the most affected by epidemics of this flavivirus. The underreporting of signs and symptoms of probable cases of dengue, the lack of characterization of the serotypes of the infection, and the few detailed studies of postmortem necropsies of patients are among other conditions that have delayed progress in the knowledge of the pathogenesis of the disease. This study presents the results of fragment sequencing assays on paraffin-embedded tissue samples from fatal DENV cases during the 2010 epidemic in Colombia. We found that the predominant serotype was DENV-2, with the Asian/American genotype of lineages 1 and 2. This work is one of the few reports of the circulating genotypes of dengue during the 2010 epidemic in Colombia, one of the most lethal dates in the country's history.
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Metadata
Title
Genotyping of dengue virus from infected tissue samples embedded in paraffin
Authors
Jorge Alonso Rivera
Aura Caterine Rengifo
Alicia Rosales-Munar
Taylor H. Díaz-Herrera
José Usme Ciro
Edgar Parra
Diego A. Alvarez-Díaz
Katherine Laiton-Donato
María Leonor Caldas
Publication date
01-12-2023
Publisher
BioMed Central
Keyword
Dengue Virus
Published in
Virology Journal / Issue 1/2023
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-023-02072-5

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