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01-03-2021 | Coronavirus | Review

Serological assays and host antibody detection in coronavirus-related disease diagnosis

Authors: Sayeh Dowlatshahi, Ehsan Shabani, Mohammad J. Abdekhodaie

Published in: Archives of Virology | Issue 3/2021

Abstract

Coronaviruses (CoV) are a family of viral pathogens that infect both birds and mammals, including humans. Seven human coronaviruses (HCoV) have been recognized so far. HCoV-229E, -OC43, -NL63, and -HKU1 account for one-third of common colds with mild symptoms. The other three members are severe acute respiratory syndrome (SARS)-CoV, Middle East respiratory syndrome (MERS)-CoV, and SARS-CoV-2. These viruses are responsible for SARS, MERS, and CoV disease 2019 (COVID-19), respectively. A variety of diagnostic techniques, including chest X-rays, computer tomography (CT) scans, analysis of viral nucleic acids, proteins, or whole virions, and host antibody detection using serological assays have been developed for the detection of these viruses. In this review, we discuss conventional serological tests, such as enzyme-linked immunosorbent assay (ELISA), western blot (WB), immunofluorescence assay (IFA), lateral flow immunoassay (LFIA), and chemiluminescence immunoassay (CLIA), as well as biosensor-based assays that have been developed for diagnosing HCoV-associated diseases since 2003, with an in-depth focus on COVID-19.

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Title: Serological assays and host antibody detection in coronavirus-related disease diagnosis
Authors: Sayeh Dowlatshahi
Ehsan Shabani
Mohammad J. Abdekhodaie
Publication date: 01-03-2021
Publisher: Springer Vienna
Keywords: Coronavirus
Severe Acute Respiratory Syndrome Coronavirus
SARS-CoV-2
Middle Eastern Respiratory Syndrome
COVID-19
Published in: Archives of Virology / Issue 3/2021
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04874-2

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