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01-12-2019 | Computed Tomography | Original Article

Imaging and histomorphometric evaluation of mandible and tibia of rats treated with bisphosphonates

Authors: Stephanie Joana Roman Martelli, Melissa Feres Damian, André Ribeiro Schinestsck, Lauren Frenzel Schuch, Andreia Morales Cascaes, Ana Carolina Uchoa Vasconcelos

Published in: Oral and Maxillofacial Surgery | Issue 4/2019

Abstract

Objectives

To evaluate the mandible and tibia of rats treated with bisphosphonates (BPs) by imaging and histomorphometric analysis.

Study design

Thirty-four rat specimens (Rattus norvegicus, Wistar strain) were distributed into 3 groups: (1) 12 rats treated with zoledronic acid; (2) 12 rats treated with clodronate; and (3) the control group, containing 10 rats that received saline. All bones were exposed to cone beam computed tomography (CBCT). The images were analyzed to determine bone density (BD), using the software OsiriX 7.0. Histological slides were prepared from the specimens and the proportion of bone volume (BV) was quantified using the software Adobe Photoshop CC.

Results

There was no statistically significant difference in BD either between the drug groups or between mandible and tibia. BV between BPs and control group did not show a significant difference. However, comparing the two bones, the mandibles in the control group displayed higher BV than did the tibiae in the same group.

Conclusion

According to our results, we conclude that (1) BD was not altered by bone type or by type of BP administered, and (2) treatment with zoledronic acid or clodronate did not affect BV in the mandible or tibia of test groups.

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Title: Imaging and histomorphometric evaluation of mandible and tibia of rats treated with bisphosphonates
Authors: Stephanie Joana Roman Martelli
Melissa Feres Damian
André Ribeiro Schinestsck
Lauren Frenzel Schuch
Andreia Morales Cascaes
Ana Carolina Uchoa Vasconcelos
Publication date: 01-12-2019
Publisher: Springer Berlin Heidelberg
Keywords: Computed Tomography
Bisphosphonate
Computed Tomography
Digital Volume Tomography
Osteonecrosis
Digital Volume Tomography
Zoledronic Acid
Published in: Oral and Maxillofacial Surgery / Issue 4/2019
Print ISSN: 1865-1550
Electronic ISSN: 1865-1569
DOI: https://doi.org/10.1007/s10006-019-00807-9

At a glance: The STEP trials

Springer Medicine

Imaging and histomorphometric evaluation of mandible and tibia of rats treated with bisphosphonates

Abstract

Objectives

Study design

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Study design

Results

Conclusion

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