Published in:
01-09-2012 | Annotated Sequence Record
Complete genome sequence of narcissus late season yellows virus infecting Chinese narcissus in China
Authors:
Shuang-Qing Lin, Jian-Guo Shen, Fang-Luan Gao, Wei Cai, Zhen Huang, Li-Yan Xie, Zu-Jian Wu
Published in:
Archives of Virology
|
Issue 9/2012
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Abstract
The complete genome sequence of a Chinese narcissus isolate of narcissus late season yellows virus from Zhangzhou, China (NLSYV-ZZ), was determined to be 9,651 nucleotides in length, excluding the 3′-terminal poly (A) tail, by amplification and sequencing of virus RNA. The viral genome contains a single long open reading frame of 9,315 nucleotides encoding a polyprotein of 3,105 amino acids. The polyprotein was predicted to be cleaved into ten mature proteins by three viral proteases. Complete genome sequence comparison and phylogenetic analysis indicated that NLSYV-ZZ was most closely related to narcissus yellow stripe virus (NYSV), which was also isolated from narcissus. These viruses shared 69.9 % identity in their complete nucleotide sequences and 77.0 % identity in their polyprotein amino acid sequences.