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Published in: European Journal of Clinical Microbiology & Infectious Diseases 5/2005

01-05-2005 | Concise Article

Comparison of genotypes of methicillin-resistant and methicillin-sensitive Staphylococcus aureus in Finland

Authors: S. Ibrahem, S. Salmenlinna, A.-M. Kerttula, A. Virolainen-Julkunen, P. Kuusela, J. Vuopio-Varkila

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 5/2005

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Abstract

The frequency of horizontal transfer of the staphylococcal cassette chromosome mec to methicillin-susceptible Staphylococcus aureus is unknown. In order to gain more information regarding this frequency in Finland, the genotypes of 299 clinical methicillin-sensitive Staphylococcus aureus isolates were compared to representatives of 24 epidemic methicillin-resistant Staphylococcus aureus genotypes. Sixty-eight percent of the methicillin-sensitive isolates had a genotype similar to eight of the epidemic methicillin-resistant strains. The remaining isolates (32%) showed 22 different genotypes. The results indicate that, in Finland, several methicillin-sensitive Staphylococcus aureus genotypes may have acquired the staphylococcal cassette chromosome mec.
Literature
1.
go back to reference Ito T, Ma XX, Takeuchi F, Okuma K, Yuzawa H, Hiramatsu K (2004) Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC. Antimicrob Agents Chemother 48:2637–2651CrossRefPubMed Ito T, Ma XX, Takeuchi F, Okuma K, Yuzawa H, Hiramatsu K (2004) Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC. Antimicrob Agents Chemother 48:2637–2651CrossRefPubMed
2.
go back to reference Fitzgerald R, Sturdevant DE, Mackie SM, Gill SR, Musser JM (2001) Evolutionary genomics of Staphylococcus aureus: insights into the origin of methicillin-resistant strain and the toxic shock syndrome epidemic. Proc Natl Acad Sci 98:8821–8826CrossRefPubMed Fitzgerald R, Sturdevant DE, Mackie SM, Gill SR, Musser JM (2001) Evolutionary genomics of Staphylococcus aureus: insights into the origin of methicillin-resistant strain and the toxic shock syndrome epidemic. Proc Natl Acad Sci 98:8821–8826CrossRefPubMed
3.
go back to reference Shukla SK, Ramaswamy SV, Conradt J, Stemper ME, Reich R, Reed KD, Graviss EA (2004) Novel polymorphisms in mec genes and a new mec complex type in methicillin-resistant Staphylococcus aureus isolates obtained in rural Wisconsin. J Antimicrob Agents Chemother 48:3080–3085CrossRef Shukla SK, Ramaswamy SV, Conradt J, Stemper ME, Reich R, Reed KD, Graviss EA (2004) Novel polymorphisms in mec genes and a new mec complex type in methicillin-resistant Staphylococcus aureus isolates obtained in rural Wisconsin. J Antimicrob Agents Chemother 48:3080–3085CrossRef
4.
go back to reference Kerttula A-M, Lyytikäinen O, Salmenlinna S, Vuopio-Varkila J (2004) Changing epidemiology of methicillin-resistant Staphylococcus aureus in Finland. J Hosp Infect 58:109–114CrossRefPubMed Kerttula A-M, Lyytikäinen O, Salmenlinna S, Vuopio-Varkila J (2004) Changing epidemiology of methicillin-resistant Staphylococcus aureus in Finland. J Hosp Infect 58:109–114CrossRefPubMed
5.
go back to reference National Committee for Clinical Laboratory Standards (2001) Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A7. NCCLS, Wayne, PA National Committee for Clinical Laboratory Standards (2001) Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A7. NCCLS, Wayne, PA
6.
go back to reference Salmenlinna S, Lyytikäinen O, Kotilainen P, Scotford R, Siren E, Vuopio-Varkila J (2000) Molecular epidemiology of methicillin-resistant Staphylococcus aureus in Finland. Eur J Clin Microbiol Infect Dis 19:101–107CrossRefPubMed Salmenlinna S, Lyytikäinen O, Kotilainen P, Scotford R, Siren E, Vuopio-Varkila J (2000) Molecular epidemiology of methicillin-resistant Staphylococcus aureus in Finland. Eur J Clin Microbiol Infect Dis 19:101–107CrossRefPubMed
7.
go back to reference Murchan S, Kaufmann ME, Deplano A, de Ryck R, Struelens M, Zinn CE, Fussing V, Salmenlinna S, Vuopio-Varkila J, EI Solh N, Cuny C, Witte W, Tassios PT, Legakis N, van Leeuwen W, van Belkum A, Vindel A, Laconcha I, Garaizar J, Haeggman S, Liljequist BO, Ransjo U, Coombes G, Cookson B (2003) Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 41:1574–1585CrossRefPubMed Murchan S, Kaufmann ME, Deplano A, de Ryck R, Struelens M, Zinn CE, Fussing V, Salmenlinna S, Vuopio-Varkila J, EI Solh N, Cuny C, Witte W, Tassios PT, Legakis N, van Leeuwen W, van Belkum A, Vindel A, Laconcha I, Garaizar J, Haeggman S, Liljequist BO, Ransjo U, Coombes G, Cookson B (2003) Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 41:1574–1585CrossRefPubMed
8.
go back to reference Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Pershing DH, Swaminathan B (1995) Guest commentary (interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239PubMed Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Pershing DH, Swaminathan B (1995) Guest commentary (interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239PubMed
9.
go back to reference Enright MC, Day NPJ, Davies CE, Peacock SJ, Spratt BG (2000) Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 38:1008–1015PubMed Enright MC, Day NPJ, Davies CE, Peacock SJ, Spratt BG (2000) Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 38:1008–1015PubMed
10.
go back to reference Wielders CLC, Vierns MR, Brisse S, de Graaf-Miltenburg LAM, Troelstra A, Fleer A, Schmitz FJ, Verhoef J, Fluit AC (2001) Evidence for in-vivo transfer of mec DNA between strains of staphylococci. Lancet 357:1674–1675CrossRefPubMed Wielders CLC, Vierns MR, Brisse S, de Graaf-Miltenburg LAM, Troelstra A, Fleer A, Schmitz FJ, Verhoef J, Fluit AC (2001) Evidence for in-vivo transfer of mec DNA between strains of staphylococci. Lancet 357:1674–1675CrossRefPubMed
11.
go back to reference Deplano A, Tassios PT, Glupczynski Y, Godfroid E, Struelens MJ (2000) In vivo deletion of the methicillin resistance mec region from the chromosome of Staphylococcus aureus strains. Antimicrob Agents Chemother 46:617–619 Deplano A, Tassios PT, Glupczynski Y, Godfroid E, Struelens MJ (2000) In vivo deletion of the methicillin resistance mec region from the chromosome of Staphylococcus aureus strains. Antimicrob Agents Chemother 46:617–619
12.
go back to reference Prevost G, Jaulhac B, Piemont Y (1992) DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J Clin Microbiol 30:967–973PubMed Prevost G, Jaulhac B, Piemont Y (1992) DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J Clin Microbiol 30:967–973PubMed
13.
go back to reference Salmenlinna S, Lyytikäinen O, Vuopio-Varkila J (2002) Community-acquired methicillin-resistant Staphylococcus aureus, Finland. Emerg Infect Dis 8:602–711PubMed Salmenlinna S, Lyytikäinen O, Vuopio-Varkila J (2002) Community-acquired methicillin-resistant Staphylococcus aureus, Finland. Emerg Infect Dis 8:602–711PubMed
Metadata
Title
Comparison of genotypes of methicillin-resistant and methicillin-sensitive Staphylococcus aureus in Finland
Authors
S. Ibrahem
S. Salmenlinna
A.-M. Kerttula
A. Virolainen-Julkunen
P. Kuusela
J. Vuopio-Varkila
Publication date
01-05-2005
Publisher
Springer-Verlag
Published in
European Journal of Clinical Microbiology & Infectious Diseases / Issue 5/2005
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-005-1328-4

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