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Digestive Diseases and Sciences
Published in: Digestive Diseases and Sciences 3/2017

01-03-2017 | Original Article

Comparative Analysis of Mutational Profile of Sonic hedgehog Gene in Gallbladder Cancer

Authors: Ruhi Dixit, Manoj Pandey, Sunil Kumar Tripathi, Amit Nandan Dhar Dwivedi, Vijay Kumar Shukla

Published in: Digestive Diseases and Sciences | Issue 3/2017

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Abstract

Background

Gallbladder cancer has high incidence in northeastern India; mortality too is high as the disease is often diagnosed late. Numerous studies have shown the role of sonic hedgehog (shh) in different cancers, an important ligand of the hedgehog signaling pathway.

Aim

This study was carried out to evaluate the shh gene mutations in gallbladder cancer patients.

Methods

PCR-SSCP was performed for shh gene in 50 samples each of gallbladder cancer, cholelithiasis, and control. The samples showing aberration in banding pattern were sequenced.

Results

Variation in banding pattern was observed in 20% gallbladder cancer cases, 10% in cholelithiasis, and none of the control (χ 2 = 11.111; p < 0.05). Sequencing results revealed seven novel point mutations in GBC cases. These novel mutations were found to be associated with histopathology (p < 0.05) and stage (p < 0.05) of gallbladder cancer.

Conclusion

This study reveals several novel individual and repetitive mutations of shh gene in GBC and cholelithiasis samples that may be used as diagnostic markers for gallbladder carcinogenesis.
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Metadata
Title
Comparative Analysis of Mutational Profile of Sonic hedgehog Gene in Gallbladder Cancer
Authors
Ruhi Dixit
Manoj Pandey
Sunil Kumar Tripathi
Amit Nandan Dhar Dwivedi
Vijay Kumar Shukla
Publication date
01-03-2017
Publisher
Springer US
Published in
Digestive Diseases and Sciences / Issue 3/2017
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI
https://doi.org/10.1007/s10620-016-4438-1

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