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World Journal of Surgical Oncology

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Open Access 01-12-2021 | Colorectal Cancer | Research

LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis

Authors: Jingfeng Gu, Liang Dong, Yun Wang, Wenjia Nie, Wencong Liu, Ji-an Zhao

Published in: World Journal of Surgical Oncology | Issue 1/2021

Abstract

Background

Long noncoding RNAs (lncRNAs) are related to colorectal cancer (CRC) development. However, the role and mechanism of lncRNA LINC01224 in CRC development are largely unknown.

Methods

LINC01224, Yin Yang 1 (YY1), microRNA (miR)-485-5p, and myosins of class VI (MYO6) levels were examined using quantitative reverse transcription polymerase chain reaction and western blotting. Functional analyses were processed through CCK-8, colony formation, flow cytometry, transwell, and xenograft analyses. Dual-luciferase reporter, chromatin immunoprecipitation (ChIP), RNA immunoprecipitation, and pull-down assays were conducted to analyze the binding interaction.

Results

LINC01224 abundance was elevated in CRC tissue samples and cell lines. Elevated LINC01224 might indicate the lower 5-year overall survival in 52 CRC patients. LINC01224 was upregulated via the transcription factor YY1. LINC01224 knockdown restrained CRC cell proliferation, migration, and invasion and increased apoptosis. MiR-485-5p was sponged by LINC01224, and miR-485-5p downregulation relieved the influence of LINC01224 interference on CRC progression. MYO6 was targeted via miR-485-5p and regulated via LINC01224/miR-485-5p axis. MiR-485-5p overexpression suppressed CRC cell proliferation, migration, and invasion and facilitated apoptosis. MYO6 upregulation mitigated the role of miR-485-5p. LINC01224 knockdown decreased xenograft tumor growth.

Conclusion

YY1-induced LINC01224 regulates CRC development via modulating miR-485-5p/MYO6 axis.

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Title: LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis
Authors: Jingfeng Gu
Liang Dong
Yun Wang
Wenjia Nie
Wencong Liu
Ji-an Zhao
Publication date: 01-12-2021
Publisher: BioMed Central
Keywords: Colorectal Cancer
Colorectal Cancer
Published in: World Journal of Surgical Oncology / Issue 1/2021
Electronic ISSN: 1477-7819
DOI: https://doi.org/10.1186/s12957-021-02389-x

2024 ASCO Annual Meeting

Springer Medicine

LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis

Abstract

Background

Methods

Results

Conclusion

2024 ASCO Annual Meeting

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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