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Published in: Archives of Virology 3/2017

01-03-2017 | Brief Report

Characterization of isolates of equine infectious anemia virus in Brazil

Authors: Dellane Martins Tigre, Camila Fonseca Lopes Brandão, Fabiana Lopes de Paula, Fabio Alexandre Chinalia, Gubio Soares Campos, Silvia Ines Sardi

Published in: Archives of Virology | Issue 3/2017

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Abstract

Equine infectious anemia is an important infectious disease that affects equids worldwide. Control of the disease is currently based on detection of anti-p26 EIAV by Agar Gel Immunodiffusion (AGID). In this work, 62 animals were examined by AGID and nested-PCR using primers for the gag gene. Fifty-three samples (85.5%) were positive by nested-PCR, whereas only 33 samples (53%) were positive for AGID. Fifteen amplicons obtained by nested-PCR were sequenced and the aligned results subjected to phylogenetic analysis. The analysis suggests that the Brazilian EIAV form a cluster with WSU5, EIAVUK and Wyoming strains from United States.
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Metadata
Title
Characterization of isolates of equine infectious anemia virus in Brazil
Authors
Dellane Martins Tigre
Camila Fonseca Lopes Brandão
Fabiana Lopes de Paula
Fabio Alexandre Chinalia
Gubio Soares Campos
Silvia Ines Sardi
Publication date
01-03-2017
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 3/2017
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-3172-5

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