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Respiratory Research
Published in: Respiratory Research 1/2022

Open Access 01-12-2022 | Bronchial Asthma | Research

Deficiency of leukocyte-specific protein 1 (LSP1) alleviates asthmatic inflammation in a mouse model

Authors: Nguyen Phuong Khanh Le, Amanda Florentina do Nascimento, David Schneberger, Chi Cuong Quach, Xiaobei Zhang, Gurpreet K. Aulakh, Wojciech Dawicki, Lixin Liu, John R. Gordon, Baljit Singh

Published in: Respiratory Research | Issue 1/2022

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Abstract

Background

Asthma is a major cause of morbidity and mortality in humans. The mechanisms of asthma are still not fully understood. Leukocyte-specific protein-1 (LSP-1) regulates neutrophil migration during acute lung inflammation. However, its role in asthma remains unknown.

Methods

An OVA-induced mouse asthma model in LSP1-deficient (Lsp1−/−) and wild-type (WT) 129/SvJ mice were used to test the hypothesis that the absence of LSP1 would inhibit airway hyperresponsiveness and lung inflammation.

Results

Light and electron microscopic immunocytochemistry and Western blotting showed that, compared with normal healthy lungs, the levels of LSP1 were increased in lungs of OVA-asthmatic mice. Compared to Lsp1−/− OVA mice, WT OVA mice had higher levels of leukocytes in broncho-alveolar lavage fluid and in the lung tissues (P < 0.05). The levels of OVA-specific IgE but not IgA and IgG1 in the serum of WT OVA mice was higher than that of Lsp1−/− OVA mice (P < 0.05). Deficiency of LSP1 significantly reduced the levels of IL-4, IL-5, IL-6, IL-13, and CXCL1 (P < 0.05) but not total proteins in broncho-alveolar lavage fluid in asthmatic mice. The airway hyper-responsiveness to methacholine in Lsp1−/− OVA mice was improved compared to WT OVA mice (P < 0.05). Histology revealed more inflammation (inflammatory cells, and airway and blood vessel wall thickening) in the lungs of WT OVA mice than in those of Lsp1−/− OVA mice. Finally, immunohistology showed localization of LSP1 protein in normal and asthmatic human lungs especially associated with the vascular endothelium and neutrophils.

Conclusion

These data show that LSP1 deficiency reduces airway hyper-responsiveness and lung inflammation, including leukocyte recruitment and cytokine expression, in a mouse model of asthma.
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Metadata
Title
Deficiency of leukocyte-specific protein 1 (LSP1) alleviates asthmatic inflammation in a mouse model
Authors
Nguyen Phuong Khanh Le
Amanda Florentina do Nascimento
David Schneberger
Chi Cuong Quach
Xiaobei Zhang
Gurpreet K. Aulakh
Wojciech Dawicki
Lixin Liu
John R. Gordon
Baljit Singh
Publication date
01-12-2022
Publisher
BioMed Central
Published in
Respiratory Research / Issue 1/2022
Electronic ISSN: 1465-993X
DOI
https://doi.org/10.1186/s12931-022-02078-7

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