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International Journal of Implant Dentistry

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Open Access 01-12-2020 | Bone Defect | Research

Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material

Authors: Guilherme dos Santos Trento, Jaqueline Suemi Hassumi, Paula Buzo Frigério, Ana Paula Farnezi Bassi, Roberta Okamoto, Marisa Aparecida Cabrini Gabrielli, Valfrido Antonio Pereira-Filho

Published in: International Journal of Implant Dentistry | Issue 1/2020

Abstract

Objective

The aim of this study is to evaluate through gene expression, immunohistochemical and microtomographic (micro-CT) analysis the response of peri-implant bone tissue around titanium implants with different surface treatments, placed in bone defects filled or not with bone substitute materials. In addition, to investigate the hypothesis that porous-hydrophilic surface induces a faster bone formation.

Materials and methods

Twenty-six animals were divided into two groups according to implant surface treatment. In each tibia, a bone defect was created followed by the placement of one implant. On the left tibia, the defect was filled with blood clot (BC), and on the right tibia, the defect was filled with biphasic hydroxyapatite/β-tricalcium-phosphate (HA/TCP) generating four subgroups: BC-N: bone defect filled with blood clot and porous surface titanium implant installed; BC-A: bone defect filled with blood clot and porous-hydrophilic surface titanium implant installed; HA/TCP-N: bone defect filled with bone substitute material and porous surface titanium implant installed; and HA/TCP-A: bone defect filled with bone substitute material and porous-hydrophilic surface titanium implant installed. The animals were submitted to euthanasia at 15, 30, and 60 days after implant installation. The expression of two genes was evaluated: RUNX2 and BSP. Immunohistochemical analyses were performed for detection of RUNX2, OPN, OCN, OPG, and RANKL antibodies and bone matrix proteins. Finally, four parameters were chosen for micro-CT analysis: trabecular number, separation and thickness, and connectivity density.

Results

Descriptive analysis showed similar findings among the experimental groups. Moreover, porous-hydrophilic surfaces presented a higher expression of RUNX2, which is probably an indicative of better osteogenesis; although the data from this study may be considered an insufficient support for a concrete statement.

Conclusion

Porous hydrophilic surface can improve and accelerate protein expression and bone formation.

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Title: Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material
Authors: Guilherme dos Santos Trento
Jaqueline Suemi Hassumi
Paula Buzo Frigério
Ana Paula Farnezi Bassi
Roberta Okamoto
Marisa Aparecida Cabrini Gabrielli
Valfrido Antonio Pereira-Filho
Publication date: 01-12-2020
Publisher: Springer Berlin Heidelberg
Keyword: Bone Defect
Published in: International Journal of Implant Dentistry / Issue 1/2020
Electronic ISSN: 2198-4034
DOI: https://doi.org/10.1186/s40729-020-00279-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material

Abstract

Objective

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Materials and methods

Results

Conclusion

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