Top

Journal of Assisted Reproduction and Genetics

Published in:

01-08-2009 | CRYOPRESERVATION

Birth of a healthy male infant after transfer of vitrified-warmed blastocysts derived from intracytoplasmic sperm injection with vitrified-warmed oocytes and frozen-thawed spermatozoa

Authors: Koichi Kyono, Yukiko Nakajo, Masakazu Doshida, Mayumi Toya, Yasuhisa Araki

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2009

Abstract

Purpose

To report a successful delivery of a healthy baby after transfer of vitrified-warmed blastocysts derived from introcytoplasmic sperm injection (ICSI) with vitrified-warmed oocytes and frozen-thawed sperm.

Methods

A female patient and her husband with non-obstructive azoospermia received a transfer of vitrified-warmed blastocysts from vitrified-warmed oocytes and frozen-thawed sperm. The main outcome measures were fertilization, pregnancy and birth.

Results

Nine oocytes were matured and vitrified. When the vitrified oocytes were warmed, six survived with good quality morphology. Using ICSI, frozen-thawed sperm was injected into the six warmed oocytes that survived, and the fertilization rate was 100%. The zygotes were cultured, and five of six early embryos became blastocysts. One of them was transferred, but pregnancy was not achieved. The second time around, two vitrified-warmed blastocysts were transferred resulting in pregnancy, and a healthy boy was delivered.

Conclusions

This is a rare case of a successful birth using a vitrified-warmed blastocyst grown after ICSI with a vitrified-warmed oocyte and frozen-thawed sperm.

Chen C. Pregnancy after human oocyte cryopreservation. Lancet. 1986;1:884–6.CrossRefPubMed

Katayama KP, Stehlik J, Kuwayama M, Kato O, Stehlik E. High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril. 2003;80:223–4.CrossRefPubMed

Kyono K, Fuchinone K, Yagi A, Nakajo Y, Yamashita A, Kumagai S. Successful pregnancy and delivery after transfer of a single blastocyst derived from a vitrified mature human oocyte. Fertil Steri. 2005;84:1017e5–6.

Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efficient vitrification method for cryopreservation of human oocytes. Reproductive BioMedicine Online. 2005;11:300–308.PubMed

Yang D, Brown SE, Nguyen K, Reddy V, Brubaker C, Winslow KL. Live birth after the transfer of human embryos developed from cryopreserved oocytes harvested before cancer treatment. Fertil Steril. 2007;87:e1–4.CrossRefPubMed

Porcu E, Venturoli S, Damiano G, Ciotti PM, Notarangelo L, Paradisi R, et al. Live birth after the transfer of human embryos developed from cryopreserved oocytes harvested before cancer treatment. Reproductive BioMedicine Online. 2008;17:267–269.

Lassalle B, Testart J, Renard JP. Human embryo features that influence the success of cryopreservation with the use of 1,2 propanediol. Fertil Steril. 1985;44:645–51.PubMed

Borini A, Bonu MA, Coticchio G, Cattoli M, Bianchi V, Cattoli M, et al. Pregnancies and birth after oocyte cryopreservation. Fertil Steril. 2004;82:601–605.CrossRefPubMed

Levi Setti PE, Albani E, Novara PV, Cesana A, Morreale G. Cryopreservation of supernumerary oocytes in IVF/ICSI cycles. Hum Reprod. 2006;22:370–375.

10.

Debra A, Gook Lyndon Hale, Edgar David H. Live birth following transfer of a cryopreserved embryo generated from a cryopreserved oocyte and cryopreserved sperm: Case report. J Assist Reprod Genet. 2007;24:43–45.CrossRef

11.

Greco E, Iacobelli M, Rienzi L, Fabris MGF, Tesoyio N, Tesarik J. Birth of a healthy boy after cryopreserved oocyte with cryopreserved testicular spermatozoa from a man with nonmasaic Klinefelter syndrome. Fertil Steril. 2008;89:991–e5–7.CrossRefPubMed

12.

Boldt J, Tidswell N, Sayers A, Kilani R, Cline D. Human oocyte cryopreservation: 5-year experience with a sodium-depleted slow freezing method. Reproductive BioMedicine Online. 2006;13:96–100.PubMedCrossRef

13.

Coticchio G, Santis LD, Rossi G, Borini A, Albertini D, Scaravelli G, et al. Sucrose concentration influences the rate of human oocytes with normal spindle and chromosome configurations after slow-cooling cryopreservation. Hum Reprod. 2006;21:1771–6.CrossRefPubMed

14.

Setti PEL, Albani E, Novara PV, Cesana A, Bianchi S, Negri L. Normal birth after transfer of cryopreserved human embryos generated by microinjection of cryopreserved testicular spermatozoa into cryopreserved human oocytes. Fertil Steril. 2005;83:1041–e9–10.

15.

Chen GA, Cai XY, Lian Y, Zheng XY, Qiao J, Chen XN, et al. Normal birth from cryopreserved embryos after intracytoplasmic sperm injection of frozen semen into vitrified human oocytes. Hum Fertil (Camb). 2008 Mar;11(1):49–51.CrossRef

16.

Montag M, Van der Ven K, Dorn C, Isachenko V, Isachenko E, van der Ven. Birth after double cryopreservation of human oocytes at metaphase II and pronuclear stages. Fertil Steril. 2006;85:751–e5–7.CrossRefPubMed

Title: Birth of a healthy male infant after transfer of vitrified-warmed blastocysts derived from intracytoplasmic sperm injection with vitrified-warmed oocytes and frozen-thawed spermatozoa
Authors: Koichi Kyono
Yukiko Nakajo
Masakazu Doshida
Mayumi Toya
Yasuhisa Araki
Publication date: 01-08-2009
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 8/2009
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-009-9338-2

At a glance: The STEP trials

Springer Medicine

Birth of a healthy male infant after transfer of vitrified-warmed blastocysts derived from intracytoplasmic sperm injection with vitrified-warmed oocytes and frozen-thawed spermatozoa

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 8/2009

Antioxidants and sperm DNA damage: a clinical perspective

From the Editor

Blastocyst transfer and gender: IVF versus ICSI

Is gestation in Prader-Willi syndrome affected by the genetic subtype?

Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10°C with electrolyte free medium

The influence of early cleavage on embryo developmental potential and IVF/ICSI outcome