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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 8/2009

01-08-2009 | Animal Experimentation in Assisted Reproduction

Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10°C with electrolyte free medium

Authors: Uttam Datta, M. Chandra Sekar, Manik Lal Hembram, Raju Dasgupta

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2009

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Abstract

Purpose

In-situ preservation of cauda epididymal spermatozoa at -10°C with electrolyte free media for obtaining maximum functional gametes than preservation at 5°C.

Methods

Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 — glycerol and soybean lecithin — Coenzyme Q10— glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5°C, wrapped with aluminium foil and freezed at — 10°C. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5°C in a water bath for 5 min.

Results

Spermatozoan characteristics were diminished gradually and significantly (p < 0.001, p < 0.05) between the media and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, viability, hypo-osmotic swelling positivity were 30.2 ± 0.62; 45.2 ± 0.82 and 41.6 ± 0.79 percent respectively on day 21.

Conclusion

This method can be adopted in field conditions for transportation of frozen epididymides and re-utilization of maximum functional gametes to conserve valuable animals after postmortem / slaughter.
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Metadata
Title
Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10°C with electrolyte free medium
Authors
Uttam Datta
M. Chandra Sekar
Manik Lal Hembram
Raju Dasgupta
Publication date
01-08-2009
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 8/2009
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-009-9344-4

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