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Chinese Medicine
Published in: Chinese Medicine 1/2022

Open Access 01-12-2022 | Atopic Dermatitis | Research

Inhibitory effect of Isatis tinctoria L. water extract on DNCB-induced atopic dermatitis in BALB/c mice and HaCaT cells

Authors: Ga-Yul Min, Tae In Kim, Ji-Hye Kim, Won-Kyung Cho, Ju-Hye Yang, Jin-Yeul Ma

Published in: Chinese Medicine | Issue 1/2022

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Abstract

Background

Isatis tinctoria L (PLG) is a medicinal herb from the roots of Isatis indigotica Fort (Family Cruciferae). Previous studies have shown that PLG has anti-inflammatory and therapeutic effects against conditions such as acute and chronic hepatitis, various respiratory inflammations, and cancer. The purpose of this study was to define the pharmacological effects of PLG on inflammatory reactions and skin hyperkeratosis, which are the main symptoms of atopic dermatitis (AD), in vivo and in vitro.

Methods

For the AD in vivo experiment, 2,4-dinitrochlorobenzene (DNCB) induction and oral administration of PLG were performed on male BALB/c mice for four weeks. For in vitro experiments, keratinocytes were activated using TNF-α/IFN-γ in cultured human keratinocyte (HaCaT) cells. PLG inhibited inflammatory chemokine production and blocked the nuclear translocation of NF-κB p65 in activated keratinocytes.

Results

As a result of oral administration of PLG, dermis and epidermis thickening, as well as eosinophil and mast cell infiltration, were attenuated in AD skin lesions. In addition, the levels of immunoglobulin E (IgE), pro-inflammatory cytokines, and the MAPK/NF-κB signaling pathway were decreased in serum and dorsal skin tissues. Furthermore, PLG inhibited inflammatory chemokine production and blocked the nuclear translocation of NF-κB p65 in activated keratinocytes. In addition, epigoitrin and adenosine, the standard compounds of PLG, were identified as candidate AD compounds.

Conclusions

These results indicate that PLG is a potent therapeutic agent for attenuating symptoms of AD.
Appendix
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Metadata
Title
Inhibitory effect of Isatis tinctoria L. water extract on DNCB-induced atopic dermatitis in BALB/c mice and HaCaT cells
Authors
Ga-Yul Min
Tae In Kim
Ji-Hye Kim
Won-Kyung Cho
Ju-Hye Yang
Jin-Yeul Ma
Publication date
01-12-2022
Publisher
BioMed Central
Published in
Chinese Medicine / Issue 1/2022
Electronic ISSN: 1749-8546
DOI
https://doi.org/10.1186/s13020-022-00624-5

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