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Lasers in Medical Science
Published in: Lasers in Medical Science 4/2014

01-07-2014 | Original Article

Apoptosis-associated genes related to photodynamic therapy in breast carcinomas

Authors: J. C. Silva, J. Ferreira-Strixino, L. C. Fontana, L. M. Paula, L. Raniero, A. A. Martin, R. A. Canevari

Published in: Lasers in Medical Science | Issue 4/2014

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Abstract

The aim of this study was to find the apoptosis molecular markers involved in the cell death that might be related to photodynamic therapy (PDT) mechanisms in breast cancer. The mammary tumors were induced in 25 Sprague-Dawley female rats by a single, oral gavage of 7,12-dimethylbenz(a)anthracene (DMBA; 70 mg/kg body weight). Animals were divided into four groups: G1 (normal, without DMBA), G2 (control, without PDT treatment), G3 (euthanized 48 h after PDT), and G4 (euthanized 24 h after PDT). For PDT experiments, the photosensitizer used was Photodithazine, and 100 J/cm of light at a fluence rate of 100 mW/cm was delivered to treat lesions. A sample of each animal was investigated by quantitative real-time PCR using Rat Apoptosis RT2 Profiler™ PCR Array platform. The results showed 20 genes with differential expression between PDT and control groups. A significant upregulation was observed for pro-apoptotic genes CASP4, CASP12, CIDEA, GADD45A, and FAS and downregulation of anti-apoptotic genes MAPK8IP1, TNFRSF11B, and NAIP2 in PDT-treated tumors. These results indicate that these genes are more directly involved in cell apoptosis induced by PDT.
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Metadata
Title
Apoptosis-associated genes related to photodynamic therapy in breast carcinomas
Authors
J. C. Silva
J. Ferreira-Strixino
L. C. Fontana
L. M. Paula
L. Raniero
A. A. Martin
R. A. Canevari
Publication date
01-07-2014
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 4/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-014-1547-y

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