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01-07-2014 | Original Article

The effects of low-level diode laser irradiation on differentiation, antigenic profile, and phagocytic capacity of osteoblast-like cells (MG-63)

Authors: Rosa Medina-Huertas, Francisco Javier Manzano-Moreno, Elvira De Luna-Bertos, Javier Ramos-Torrecillas, Olga García-Martínez, Concepción Ruiz

Published in: Lasers in Medical Science | Issue 4/2014

Abstract

Previous in vivo and in vitro studies have reported that low-level diode laser therapy induces a biostimulatory effect, such as cell proliferation. The aim of the present study was to evaluate whether the laser irradiation of osteoblast-like cells (MG-63) can modify alkaline phosphatase activity (ALP), antigenic profile, and phagocytic capacity. The MG-63 cell line was exposed to diode laser (ezLase) of 940 nm at 1–1.5 W/cm² and 3–4 J. ALP was evaluated by a spectrophotometric technique and antigenic expression analysis (CD 54, CD80, CD86, HLA-DR), and phagocytic activity was analyzed by flow cytometry. At 24 h, the treated groups showed an increased ALP, and the highest increase versus controls (P = 0.002) was at the dose of 1 W/cm² and 3 J; this modulation of the antigenic profile translated into a reduced expression of CD54, CD86, and HLA-DR and a slightly decreased phagocytic capacity with respect to the nonirradiated control group at the different intensities and fluencies assayed. These results demonstrate that laser therapy can exert a biostimulatory effect on osteoblastic cells at different levels, which may be clinically useful in the regeneration of bone tissue.

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Title: The effects of low-level diode laser irradiation on differentiation, antigenic profile, and phagocytic capacity of osteoblast-like cells (MG-63)
Authors: Rosa Medina-Huertas
Francisco Javier Manzano-Moreno
Elvira De Luna-Bertos
Javier Ramos-Torrecillas
Olga García-Martínez
Concepción Ruiz
Publication date: 01-07-2014
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 4/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1557-9

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The effects of low-level diode laser irradiation on differentiation, antigenic profile, and phagocytic capacity of osteoblast-like cells (MG-63)

Abstract

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Abstract

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