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01-02-2021 | Alzheimer's Disease | Original Article

Physical exercise prevents amyloid β₁₋₄₀-induced disturbances in NLRP3 inflammasome pathway in the hippocampus of mice

Authors: Julia M. Rosa, Anderson Camargo, Ingrid A. V. Wolin, Manuella P. Kaster, Ana Lúcia S. Rodrigues

Published in: Metabolic Brain Disease | Issue 2/2021

Abstract

Amyloid beta (Aβ), one of the main hallmarks of Alzheimer’s Disease (AD), may stimulate pattern recognition receptors (PRR) such as the NLRP3 inflammasome, inducing a pro-inflammatory state in the brain that contributes to disease development. Physical exercise can have multiple beneficial effects on brain function, including anti-inflammatory and neuroprotective roles. The objective of this study was to investigate the prophylactic effect of moderate treadmill exercise for 4 weeks on inflammatory events related to NLRP3 signaling in the hippocampus of mice after intracerebroventricular Aβ₁₋₄₀ administration. Our results show that Aβ₁₋₄₀ administration (400 pmol/mouse, i.c.v.) significantly increased the immunocontent Iba-1 (a microglial reactivity marker), NLRP3, TXNIP, and caspase-1 in the hippocampus of mice. However, physical exercise prevented the hippocampal increase in Iba-1, TXNIP, and activation of the NLRP3 inflammasome pathway caused by Aβ₁₋₄₀. Moreover, physical exercise per se reduced the TXNIP and caspase-1 immunocontent in the hippocampus. No alterations were observed on the immunocontent of GFAP, ASC, and IL-1β in the hippocampus after Aβ₁₋₄₀ and/or physical exercise. These results reinforce the role of NLRP3 inflammasome pathway in AD and point to physical exercise as a possible non-pharmacological strategy to prevent inflammatory events triggered by Aβ₁₋₄₀ in mice.

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Title: Physical exercise prevents amyloid β1−40-induced disturbances in NLRP3 inflammasome pathway in the hippocampus of mice
Authors: Julia M. Rosa
Anderson Camargo
Ingrid A. V. Wolin
Manuella P. Kaster
Ana Lúcia S. Rodrigues
Publication date: 01-02-2021
Publisher: Springer US
Keywords: Alzheimer's Disease
Alzheimer's Disease
Published in: Metabolic Brain Disease / Issue 2/2021
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-020-00646-8

At a glance: The STEP trials

Springer Medicine

Physical exercise prevents amyloid β₁₋₄₀-induced disturbances in NLRP3 inflammasome pathway in the hippocampus of mice

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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