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BMC Immunology
Published in: BMC Immunology 1/2012

Open Access 01-12-2012 | Research article

Adjuvant effect of docetaxel on the immune responses to influenza A H1N1 vaccine in mice

Authors: Jian Chen, Lin Yuan, Qing Fan, Fei Su, Yu Chen, Songhua Hu

Published in: BMC Immunology | Issue 1/2012

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Abstract

Background

Vaccination remains one of the most effective approaches to prevent the spread of infectious diseases. Immune responses to vaccination can be enhanced by inclusion of adjuvant in a vaccine. Paclitaxel extracted from the bark of the Pacific yew tree Taxus brevifola was previously demonstrated to have adjuvant property. Compared to paclitaxel, docetaxel is another member of taxane family, and is more soluble in water and easier to manipulate in medication. To investigate the adjuvant effect of this compound, we measured the immune responses induced by co-administration of a split inactivated influenza H1N1 vaccine antigen with docetaxel.

Results

When co-administered with docetaxel, lower dose antigen (equivalent to 10 ng HA) induced similar levels of IgG and IgG isotypes as well as HI titers to those induced by higher dose antigen (equivalent to 100 ng HA). Docetaxel promoted splenocyte responses to H1N1 antigen, ConA and LPS, mRNA expressions of cytokines (IFN-gamma, IL-12, IL-4 and IL-10) and T-bet/GATA-3 by splenocytes. The enhanced immunity was associated with up-expressed microRNAs (miR-155, miR-150 and miR-146a) in docetaxel-stimulated RAW264.7 cells. Docetaxel promoted similar IgE level to but alum promoted significantly higher IgE level than the control.

Conclusion

Docetaxel has adjuvant effect on the influenza H1N1 vaccine by up-regulation of Th1/Th2 immune responses. Considering its unique vaccine adjuvant property as well as the safe record as an anti-neoplastic agent clinically used in humans during a long period, docetaxel should be further studied for its use in influenza vaccine production.
Appendix
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Literature
1.
Barry DW, Mayner RE, Staton E, Dunlap RC, Rastogi SC, Hannah JE, Blackburn RJ, Nortman DF, Graze PR: Comparative trial of influenza vaccines. I. Immunogenicity of whole virus and split product vaccines in man. Am J Epidemiol. 1976, 104: 34-46.PubMed
2.
3.
Ferguson NM, Cummings DA, Fraser C, Cajka JC, Cooley PC, Burke DS: Strategies for mitigating an influenza pandemic. Nature. 2006, 442: 448-452. 10.1038/nature04795.PubMedCrossRef
4.
Hancock K, Veguilla V, Lu X, Zhong W, Butler EN, Sun H, Liu F, Dong L, DeVos JR, Gargiullo PM, Brammer TL, Cox NJ, Tumpey TM, Katz JM: Cross-reactive antibody responses to the 2009 pandemic H1N1 influenza virus. N Engl J Med. 2009, 361: 1945-1952. 10.1056/NEJMoa0906453.PubMedCrossRef
5.
Kieny MP, Costa A, Hombach J, Carrasco P, Pervikov Y, Salisbury D, Greco M, Gust I, LaForce M, Franco-Paredes C, Santos JI, D’Hondt E, Rimmelzwaan G, Karron R, Fukuda K: A global pandemic influenza vaccine action plan. Vaccine. 2006, 24: 6367-6370. 10.1016/j.vaccine.2006.07.021.PubMedCrossRef
6.
7.
Hu AY, Weng TC, Tseng YF, Chen YS, Wu CH, Hsiao S, Chou AH, Chao HJ, Gu A, Wu SC, Chong P, Lee MS: Microcarrier-based MDCK cell culture system for the production of influenza H5N1 vaccines. Vaccine. 2008, 26: 5736-5740. 10.1016/j.vaccine.2008.08.015.PubMedCrossRef
8.
9.
Kistner O, Howard MK, Spruth M, Wodal W, Bruhl P, Gerencer M, Crowe BA, Savidis-Dacho H, Livey I, Reiter M, Mayerhofer I, Tauer C, Grillberger L, Mundt W, Falkner FG, Barrett PN: Cell culture (Vero) derived whole virus (H5N1) vaccine based on wild-type virus strain induces cross-protective immune responses. Vaccine. 2007, 25: 6028-6036. 10.1016/j.vaccine.2007.05.013.PubMedPubMedCentralCrossRef
10.
Ninomiya A, Imai M, Tashiro M, Odagiri T: Inactivated influenza H5N1 whole-virus vaccine with aluminum adjuvant induces homologous and heterologous protective immunities against lethal challenge with highly pathogenic H5N1 avian influenza viruses in a mouse model. Vaccine. 2007, 25: 3554-3560. 10.1016/j.vaccine.2007.01.083.PubMedCrossRef
11.
Gupta RK: Aluminum compounds as vaccine adjuvants. Adv Drug Deliv Rev. 1998, 32: 155-172. 10.1016/S0169-409X(98)00008-8.PubMedCrossRef
12.
Podda A, Giudice GD: MF59-adjuvanted vaccines: increased immunogenicity with an optimal safety profile. Expert Rev Vaccines. 2003, 2: 197-203. 10.1586/14760584.2.2.197.PubMedCrossRef
13.
Podda A: The adjuvanted influenza vaccines with novel adjuvants: experience with the MF59-adjuvanted vaccine. Vaccine. 2001, 19: 2673-2680. 10.1016/S0264-410X(00)00499-0.PubMedCrossRef
14.
Parretta E, Ianniello B, Ferrazin F, Rossi F, Capuano A: Italian post-marketing surveillance for adverse event reports after MF59-adjuvanted H1N1v vaccination. Vaccine. 2011, 29: 3708-3713. 10.1016/j.vaccine.2011.02.097.PubMedCrossRef
15.
Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT: Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Am Chem Soc. 1971, 93: 2325-2327. 10.1021/ja00738a045.PubMedCrossRef
16.
Schiff PB, Fant J, Horwitz SB: Promotion of microtubule assembly in vitro by taxol. Nature. 1979, 277: 665-667. 10.1038/277665a0.PubMedCrossRef
17.
Abal M, Andreu JM, Barasoain I: Taxanes: microtubule and centrosome targets, and cell cycle dependent mechanisms of action. Curr Cancer Drug Targets. 2003, 3: 193-203. 10.2174/1568009033481967.PubMedCrossRef
18.
Tankanow RM: Docetaxel: a taxoid for the treatment of metastatic breast cancer. Am J Health Syst Pharm. 1998, 55: 1777-1791.PubMed
19.
Rowinsky EK, Donehower RC: Paclitaxel (taxol). N Engl J Med. 1995, 332: 1004-1014. 10.1056/NEJM199504133321507.PubMedCrossRef
20.
Byrd-Leifer CA, Block EF, Takeda K, Akira S, Ding A: The role of MyD88 and TLR4 in the LPS-mimetic activity of Taxol. Eur J Immunol. 2001, 31: 2448-2457. 10.1002/1521-4141(200108)31:8<2448::AID-IMMU2448>3.0.CO;2-N.PubMedCrossRef
21.
Yuan L, Wu L, Chen J, Wu Q, Hu S: Paclitaxel acts as an adjuvant to promote both Th1 and Th2 immune responses induced by ovalbumin in mice. Vaccine. 2010, 28: 4402-4410. 10.1016/j.vaccine.2010.04.046.PubMedCrossRef
22.
Garnett CT, Schlom J, Hodge JW: Combination of docetaxel and recombinant vaccine enhances T-cell responses and antitumor activity: effects of docetaxel on immune enhancement. Clin Cancer Res. 2008, 14: 3536-3544. 10.1158/1078-0432.CCR-07-4025.PubMedPubMedCentralCrossRef
23.
Cox RJ, Hovden AO, Brokstad KA, Szyszko E, Madhun AS, Haaheim LR: The humoral immune response and protective efficacy of vaccination with inactivated split and whole influenza virus vaccines in BALB/c mice. Vaccine. 2006, 24: 6585-6587. 10.1016/j.vaccine.2006.05.040.PubMedCrossRef
24.
Caillet C, Piras F, Bernard MC, de Montfort A, Boudet F, Vogel FR, Hoffenbach A, Moste C, Kusters I: AF03-adjuvanted and non-adjuvanted pandemic influenza A (H1N1) 2009 vaccines induce strong antibody responses in seasonal influenza vaccine-primed and unprimed mice. Vaccine. 2010, 28: 3076-3079. 10.1016/j.vaccine.2010.02.050.PubMedCrossRef
25.
Nauta JJP, Bruijn IA: On the bias in HI titers and how to reduce it. Vaccine. 2006, 24: 6645-6646. 10.1016/j.vaccine.2006.05.052.PubMedCrossRef
26.
Gross PA, Davis AE: Neutralization test in influenza: use in individuals without hemagglutination inhibition antibody. J Clin Microbiol. 1979, 10: 382-384.PubMedPubMedCentral
27.
Crume KP, O’Sullivan D, Miller JH, Northcote PT, La Flamme AC: Delaying the onset of experimental autoimmune encephalomyelitis with the microtubule-stabilizing compounds, paclitaxel and Peloruside A. J Leukoc Biol. 2009, 86: 949-958. 10.1189/jlb.0908541.PubMedCrossRef
28.
O’Connell RM, Taganov KD, Boldin MP, Cheng G, Baltimore D: MicroRNA-155 is induced during the macrophage inflammatory response. Proc Natl Acad Sci U S A. 2007, 104: 1604-1609. 10.1073/pnas.0610731104.PubMedPubMedCentralCrossRef
29.
Xiao C, Calado DP, Galler G, Thai TH, Patterson HC, Wang J, Rajewsky N, Bender TP, Rajewsky K: MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell. 2007, 131: 146-159. 10.1016/j.cell.2007.07.021.PubMedCrossRef
30.
Chen CZ, Li L, Lodish HF, Bartel DP: MicroRNAs modulate hematopoietic lineage differentiation. Science. 2004, 303: 83-86. 10.1126/science.1091903.PubMedCrossRef
31.
Li QJ, Chau J, Ebert PJ, Sylvester G, Min H, Liu G, Braich R, Manoharan M, Soutschek J, Skare P, Klein LO, Davis MM, Chen CZ: miR-181a is an intrinsic modulator of T cell sensitivity and selection. Cell. 2007, 129: 147-161. 10.1016/j.cell.2007.03.008.PubMedCrossRef
32.
Verweij J, Clavel M, Chevalier B: Paclitaxel (Taxol) and docetaxel (Taxotere): not simply two of a kind. Ann Oncol. 1994, 5: 495-505.PubMed
33.
Bissery MC, Nohynek G, Sanderink GJ, Lavelle F: Docetaxel (Taxotere): a review of preclinical and clinical experience. Part I: Preclinical experience. Anticancer Drugs. 1995, 6: 339-355. 10.1097/00001813-199506000-00001. 363–8PubMedCrossRef
34.
Hehme N, Engelmann H, Kuenzel W, Neumeier E, Saenger R: Immunogenicity of a monovalent, aluminum-adjuvanted influenza whole virus vaccine for pandemic use. Virus Res. 2004, 103: 163-171. 10.1016/j.virusres.2004.02.029.PubMedCrossRef
35.
Hamaoka T, Katz DH, Benacerraf B: Hapten-specific IgE antibody responses in mice. II. Cooperative interactions between adoptively transferred T and B lymphocytes in the development of IgE response. J Exp Med. 1973, 138: 538-556. 10.1084/jem.138.3.538.PubMedPubMedCentralCrossRef
36.
Seitz CS, Brocker EB, Trautmann A: Vaccination-associated anaphylaxis in adults: diagnostic testing ruling out IgE-mediated vaccine allergy. Vaccine. 2009, 27: 3885-3889. 10.1016/j.vaccine.2009.04.020.PubMedCrossRef
37.
Wood JM, Schild GC, Newman RW, Seagroatt V: An improved single-radial-immunodiffusion technique for the assay of influenza haemagglutinin antigen: application for potency determinations of inactivated whole virus and subunit vaccines. J Biol Stand. 1977, 5: 237-247. 10.1016/S0092-1157(77)80008-5.PubMedCrossRef
38.
Song X, Chen J, Sakwiwatkul K, Li R, Hu S: Enhancement of immune responses to influenza vaccine (H3N2) by ginsenoside Re. Int Immunopharmacol. 2010, 10: 351-356. 10.1016/j.intimp.2009.12.009.PubMedCrossRef
39.
Kendal AP, Pereira MS, Skehel JJ: Hemagglutination inhibition. Concepts and procedures for laboratory-based influenza surveillance. Edited by: Kendal AP, Pereira MS, Skehel JJ. 1982, Centers for Disease Control and Prevention and Pan-American Health Organization, Atlanta, B17-B35.
40.
Sun J, Hu S, Song X: Adjuvant effects of protopanaxadiol and protopanaxatriol saponins from ginseng roots on the immune responses to ovalbumin in mice. Vaccine. 2007, 25: 1114-1120. 10.1016/j.vaccine.2006.09.054.PubMedCrossRef
41.
Bustin SA: Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol. 2000, 25: 169-193. 10.1677/jme.0.0250169.PubMedCrossRef
42.
Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 2001, 25: 402-408. 10.1006/meth.2001.1262.PubMedCrossRef
43.
Purcell MK, Kurath G, Garver KA, Herwig RP, Winton JR: Quantitative expression profiling of immune response genes in rainbow trout following infectious haematopoietic necrosis virus (IHNV) infection or DNA vaccination. Fish Shellfish Immunol. 2004, 17: 447-462. 10.1016/j.fsi.2004.04.017.PubMedCrossRef
Metadata
Title
Adjuvant effect of docetaxel on the immune responses to influenza A H1N1 vaccine in mice
Authors
Jian Chen
Lin Yuan
Qing Fan
Fei Su
Yu Chen
Songhua Hu
Publication date
01-12-2012
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2012
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/1471-2172-13-36

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