Top

Journal of Assisted Reproduction and Genetics

Published in:

01-11-2007 | Original Paper

Visualization of meiotic spindle and subsequent embryonic development in in vitro and in vivo matured human oocytes

Authors: Cong Fang, Mandy Tang, Tao Li, Wen-Lin Peng, Can-Quan Zhou, Guang-Lun Zhuang, Milton Leong

Published in: Journal of Assisted Reproduction and Genetics | Issue 11/2007

Abstract

Purpose

The aim of this study is to investigate the relationship between spindle location and embryonic development of in vivo and in vitro matured human oocytes.

Methods

The spindles of 134 in vivo matured, 105 in vitro matured oocytes were examined by Polscope at the time of ICSI.

Results

The spindles were visualized in 83.6 and 77.1% of in vivo and in vitro matured oocytes respectively. The rate of fertilization of in vivo matured oocytes with spindles beneath or adjacent to the first polar body (angle of 0–5°) was significantly higher (93.3%) than all other groups. The proportions of various spindle positions did not differ statistically in in vivo and in vitro matured oocytes.

Conclusions

Meiotic spindle location with regard to the first polar body appears to influence fertilization rate.

Liu L, Oldenbourg R, Trimarchi JR, Keefe DL. A reliable, non-invasive technique for spindle imaging and enucleation of mammalian oocytes. Nat Biotechnol 2000;18:223–5.PubMedCrossRef

Wang WH, Meng L, Hackett RJ, Odenbourg R, Keefe KL. The spindle observation and its relationship with fertilization after ICSI in living human oocytes. Fertil Steril 2001;75:348–53.PubMedCrossRef

Wang WH, Meng L, Hackett RJ, Keefe DL. Development ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination. Hum Reprod 2001;16:1464–8.PubMedCrossRef

Rienzi L, Ubaldi F, Marinez F, Iacobeli M, Minasi MG, Ferrero S, et al. Relationship between meiotic spindle location with regard to the polar body positon and oocyte developmental potential after ICSI. Hum Reprod 2003;18:1289–93.PubMedCrossRef

Moon JH, Jee BC, Ku SY, Suh CS, Kim SH, Choi YM, et al. Spindle positions and their distributions in in vivo and in vitro matured mouse oocytes. Hum Reprod 2005;20:2207–10.PubMedCrossRef

Battaglia DE, Goodwin P, Klein NA, Soules MR. Influence of maternal age on meiotic spindle in oocytes from naturally cycling women. Hum Reprod 1996;11:2217–22.PubMed

Cooke S, Tyler JPP, Driscoll GL. Meiotic spindle location and identification and its effect on embryonic cleavage plane and early development. Hum Reprod 2003;18:2397–405.PubMedCrossRef

Konc J, Kanyó K, Cseh S. Visualization and examination of the meiotic spindle in human oocytes with Polscope. J Assist Reprod Genet 2003;21:349–53.CrossRef

Dvorak M, Tesarid J. Ultrastructure of human ovarian follicles. In: Motta PM, Hafez ESE, editors. Biology of the ovary. The Hague: Martinus Nijhoff; 1980. p. 121–37.

10.

Moon JH, Hyun CS, Lee SW, Son WY, Yoon SH, Lim JH. Visualization of the metaphase II meiotic spindle in living human oocytes using Polscope enables the prediction of embryonic developmental competence after ICSI. Hum Reprod 2003;18:817–20.PubMedCrossRef

Title: Visualization of meiotic spindle and subsequent embryonic development in in vitro and in vivo matured human oocytes
Authors: Cong Fang
Mandy Tang
Tao Li
Wen-Lin Peng
Can-Quan Zhou
Guang-Lun Zhuang
Milton Leong
Publication date: 01-11-2007
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 11/2007
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-007-9171-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Visualization of meiotic spindle and subsequent embryonic development in in vitro and in vivo matured human oocytes

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 11/2007

Cytogenetic testing of anembryonic pregnancies compared to embryonic missed abortions

Transvaginal ultrasound assessment of the premenopausal ovarian mass

Cytokines: Important for implantation?

JARG blog

PTEN and Akt expression during growth of human ovarian follicles

The incidence of aberrant endometrial αvβ3 vitronectin expression in a high risk infertility population: could prolonged GnRH agonist therapy play a role?