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Journal of Assisted Reproduction and Genetics

Published in:

01-11-2007 | Physiology

PTEN and Akt expression during growth of human ovarian follicles

Authors: Maki Goto, Akira Iwase, Hisao Ando, Shozo Kurotsuchi, Toko Harata, Fumitaka Kikkawa

Published in: Journal of Assisted Reproduction and Genetics | Issue 11/2007

Abstract

Purpose

To assess the expression of PTEN and total and phosphorylated Akt in human ovarian follicles during follicular growth.

Methods

Immunohistochemistry of ovarian tissues and Western blotting and immunofluorescence of primary cultured luteinized granulosa cells for PTEN and Akt.

Results

Immunoreactivity of Akt was found in the oocytes, granulosa cells and theca cells in primordial follicles, follicles at each growing stage and luteal cells. As the follicles grew, staining for PTEN became intense in the granulosa cells, whereas the intensity of phospho-Akt became weak. Western blotting and immunofluorescence analysis using primary cultured granulosa-lutein cells showed Akt and PTEN expression, and phosphorylation of Akt in vitro.

Conclusions

PTEN and Akt are present in the granulosa cells during folliculogenesis. An increase in PTEN may lead to changes in proliferation and/or differentiation of granulosa cells during follicular growth via regulation of Akt phosphorylation.

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Title: PTEN and Akt expression during growth of human ovarian follicles
Authors: Maki Goto
Akira Iwase
Hisao Ando
Shozo Kurotsuchi
Toko Harata
Fumitaka Kikkawa
Publication date: 01-11-2007
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 11/2007
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-007-9156-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

PTEN and Akt expression during growth of human ovarian follicles

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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