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Virology Journal
Published in: Virology Journal 1/2008

Open Access 01-12-2008 | Research

Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture

Authors: Hironori Yoshii, Kay Sadaoka, Masaaki Matsuura, Kazuhiro Nagaike, Michiaki Takahashi, Koichi Yamanishi, Yasuko Mori

Published in: Virology Journal | Issue 1/2008

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Abstract

Background

Open reading frame 58 (ORF58) of varicella-zoster virus (VZV) lies at the 3'end of the Unique long (UL) region and its functional is unknown. In order to clarify whether ORF58 is essential for the growth of VZV, we constructed a deletion mutant of ORF58 (pOka-BACΔ58) from the Oka parental genome cloned into a bacterial artificial chromosome (pOka-BAC).

Results

The ORF58-deleted virus (rpOkaΔ58) was reconstituted from the pOka-BACΔ58 genome in MRC-5 cells, indicating that the ORF58 gene is non-essential for virus growth. Comparison of the growth rate of rpOkaΔ58 and recombinant wild-type virus by assessing plaque sizes revealed no significant differences between them both in MRC-5 cells and malignant melanoma cells.

Conclusion

This study shows that the ORF58 gene is dispensable for viral replication and does not affect the virus' ability to form plaques in vitro.
Appendix
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Literature
1.
2.
Cohen JI, Straus SE, Arvin AM: Varicella-Zoster Virus Replication, Pathogenesis, and Management. In Fields VIROLOGY. 5th edition. Edited by: Knipe D, Griffin D, Lamb R, Straus S, Howley P, Martin M, Roizman B. Philadelphia: Lippincott Williams & Wilkins, a Wolters Kluwer Business; 2007:2773-2818.
3.
Cox E, Reddy S, Iofin I, Cohen JI: Varicella-zoster virus ORF57, unlike its pseudorabies virus UL3.5 homolog, is dispensable for viral replication in cell culture. Virology 1998,250(1):205-209. 10.1006/viro.1998.9349CrossRefPubMed
4.
Brune W, Messerle M, Koszinowski UH: Forward with BACs: new tools for herpesvirus genomics. Trends Genet 2000,16(6):254-259. 10.1016/S0168-9525(00)02015-1CrossRefPubMed
5.
Nagaike K, Mori Y, Gomi Y, Yoshii H, Takahashi M, Wagner M, Koszinowski U, Yamanishi K: Cloning of the varicella-zoster virus genome as an infectious bacterial artificial chromosome in Escherichia coli. Vaccine 2004,22(29–30):4069-4074. 10.1016/j.vaccine.2004.03.062CrossRefPubMed
6.
Narayanan K, Williamson R, Zhang Y, Stewart AF, Ioannou PA: Efficient and precise engineering of a 200 kb beta-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system. Gene Ther 1999,6(3):442-447. 10.1038/sj.gt.3300901CrossRefPubMed
7.
Kanegae Y, Lee G, Sato Y, Tanaka M, Nakai M, Sakaki T, Sugano S, Saito I: Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res 1995,23(19):3816-3821. 10.1093/nar/23.19.3816PubMedCentralCrossRefPubMed
8.
Moffat JF, Zerboni L, Kinchington PR, Grose C, Kaneshima H, Arvin AM: Attenuation of the vaccine Oka strain of varicella-zoster virus and role of glycoprotein C in alphaherpesvirus virulence demonstrated in the SCID-hu mouse. J Virol 1998,72(2):965-974.PubMedCentralPubMed
9.
Moffat JF, Zerboni L, Sommer MH, Heineman TC, Cohen JI, Kaneshima H, Arvin AM: The ORF47 and ORF66 putative protein kinases of varicella-zoster virus determine tropism for human T cells and skin in the SCID-hu mouse. Proc Natl Acad Sci USA 1998,95(20):11969-11974. 10.1073/pnas.95.20.11969PubMedCentralCrossRefPubMed
10.
Sato B, Ito H, Hinchliffe S, Sommer MH, Zerboni L, Arvin AM: Mutational analysis of open reading frames 62 and 71, encoding the varicella-zoster virus immediate-early transactivating protein, IE62, and effects on replication in vitro and in skin xenografts in the SCID-hu mouse in vivo. J Virol 2003,77(10):5607-5620. 10.1128/JVI.77.10.5607-5620.2003PubMedCentralCrossRefPubMed
11.
Besser J, Sommer MH, Zerboni L, Bagowski CP, Ito H, Moffat J, Ku CC, Arvin AM: Differentiation of varicella-zoster virus ORF47 protein kinase and IE62 protein binding domains and their contributions to replication in human skin xenografts in the SCID-hu mouse. J Virol 2003,77(10):5964-5974. 10.1128/JVI.77.10.5964-5974.2003PubMedCentralCrossRefPubMed
12.
Baiker A, Fabel K, Cozzio A, Zerboni L, Fabel K, Sommer M, Uchida N, He D, Weissman I, Arvin AM: Varicella-zoster virus infection of human neural cells in vivo. Proc Natl Acad Sci USA 2004,101(29):10792-10797. 10.1073/pnas.0404016101PubMedCentralCrossRefPubMed
13.
Moffat J, Mo C, Cheng JJ, Sommer M, Zerboni L, Stamatis S, Arvin AM: Functions of the C-terminal domain of varicella-zoster virus glycoprotein E in viral replication in vitro and skin and T-cell tropism in vivo. J Virol 2004,78(22):12406-12415. 10.1128/JVI.78.22.12406-12415.2004PubMedCentralCrossRefPubMed
14.
Ito H, Sommer MH, Zerboni L, Baiker A, Sato B, Liang R, Hay J, Ruyechan W, Arvin AM: Role of the varicella-zoster virus gene product encoded by open reading frame 35 in viral replication in vitro and in differentiated human skin and T cells in vivo. J Virol 2005,79(8):4819-4827. 10.1128/JVI.79.8.4819-4827.2005PubMedCentralCrossRefPubMed
15.
Schaap A, Fortin JF, Sommer M, Zerboni L, Stamatis S, Ku CC, Nolan GP, Arvin AM: T-cell tropism and the role of ORF66 protein in pathogenesis of varicella-zoster virus infection. J Virol 2005,79(20):12921-12933. 10.1128/JVI.79.20.12921-12933.2005PubMedCentralCrossRefPubMed
16.
Berarducci B, Sommer M, Zerboni L, Rajamani J, Arvin AM: Cellular and viral factors regulate the varicella-zoster virus gE promoter during viral replication. J Virol 2007,81(19):10258-10267. 10.1128/JVI.00553-07PubMedCentralCrossRefPubMed
17.
Che X, Berarducci B, Sommer M, Ruyechan WT, Arvin AM: The ubiquitous cellular transcriptional factor USF targets the varicella-zoster virus open reading frame 10 promoter and determines virulence in human skin xenografts in SCIDhu mice in vivo. J Virol 2007,81(7):3229-3239. 10.1128/JVI.02537-06PubMedCentralCrossRefPubMed
18.
Zhang Z, Rowe J, Wang W, Sommer M, Arvin A, Moffat J, Zhu H: Genetic Analysis of Varicella-Zoster Virus ORF0 to ORF4 by Use of a Novel Luciferase Bacterial Artificial Chromosome System. J Virol 2007,81(17):9024-9033. 10.1128/JVI.02666-06PubMedCentralCrossRefPubMed
19.
Ghiasi H, Perng GC, Cai S, Nesburn AB, Wechsler SL: The UL3 open reading frame of herpes simplex virus type 1 codes for a phosphoprotein. Virus Res 1996,44(2):137-142. 10.1016/0168-1702(96)01330-5CrossRefPubMed
20.
Yamada H, Jiang YM, Zhu HY, Inagaki-Ohara K, Nishiyama Y: Nucleolar localization of the UL3 protein of herpes simplex virus type 2. J Gen Virol 1999,80(Pt 8):2157-2164.CrossRefPubMed
Metadata
Title
Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture
Authors
Hironori Yoshii
Kay Sadaoka
Masaaki Matsuura
Kazuhiro Nagaike
Michiaki Takahashi
Koichi Yamanishi
Yasuko Mori
Publication date
01-12-2008
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2008
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-5-54

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