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Cancer and Metastasis Reviews
Published in: Cancer and Metastasis Reviews 1/2016

01-03-2016

Use of fluorescent proteins and color-coded imaging to visualize cancer cells with different genetic properties

Author: Robert M. Hoffman

Published in: Cancer and Metastasis Reviews | Issue 1/2016

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Abstract

Fluorescent proteins are very bright and available in spectrally-distinct colors, enable the imaging of color-coded cancer cells growing in vivo and therefore the distinction of cancer cells with different genetic properties. Non-invasive and intravital imaging of cancer cells with fluorescent proteins allows the visualization of distinct genetic variants of cancer cells down to the cellular level in vivo. Cancer cells with increased or decreased ability to metastasize can be distinguished in vivo. Gene exchange in vivo which enables low metastatic cancer cells to convert to high metastatic can be color-coded imaged in vivo. Cancer stem-like and non-stem cells can be distinguished in vivo by color-coded imaging. These properties also demonstrate the vast superiority of imaging cancer cells in vivo with fluorescent proteins over photon counting of luciferase-labeled cancer cells.
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Metadata
Title
Use of fluorescent proteins and color-coded imaging to visualize cancer cells with different genetic properties
Author
Robert M. Hoffman
Publication date
01-03-2016
Publisher
Springer US
Published in
Cancer and Metastasis Reviews / Issue 1/2016
Print ISSN: 0167-7659
Electronic ISSN: 1573-7233
DOI
https://doi.org/10.1007/s10555-016-9610-8

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