Published in:
01-06-2000 | Non-peer-reviewed research
Type V collagen induces apoptosis of 8701-BC breast cancer cells and enhances m-calpain expression
Published in: Breast Cancer Research | Issue 3/2000
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Introduction
We previously reported that ductal infiltrating carcinomas (DIC) of the human breast display profound modifications of the stromal architecture, associated with anomalous collagen composition. The major alterations observed in the interstitial collagen were an abnormal ratio between type I and type III collagens, the appearence of an onco-foetal form of collagen (OF/LB) and a relative increase of type V collagen content. Biological assays performed by culturing a DIC-derived cell line (8701-BC) onto type V collagen substrate demonstrated that the latter was able to restrain cell growth and to inhibit cell motility and invasion "in vitro", differently from what found with other collagen species tested.
Aims
To search for molecular mechanisms underlying the observed inhibitory effect of collagen type V on breast cancer cells. As a reference model, we used culture substrates prepared with type IV collagen, which represents the physiological substrate for cells of epithelial origin.
Methods
Apoptosis was studied by both fluorescence microscopical analyses of cell viability and DNA fragmentation assays in preparations of 8701-BC cells grown onto either type V and type IV collagen. Differences in gene expression following cell adhesion onto the two substrates were analyzed by a "differential display" PCR (DD-PCR) technique and Western blot.
Results
In this paper we demonstrate that the inhibitory effect exerted by type V collagen is consistently associated with the switching-on of a death program by a significant proportion of the cell culture, concomitant with the formation of cohesive cell islands displaying a progressive decrease of cell spreading. DD-PCR and Western blot assays demonstrated a consistent association of type V collagen-promoted apoptosis with the up-regulation of the large subunit of m-calpain (L-mC) at both mRNA and protein level. Cell exposure to calpain inibitor I decreased the amount of DNA fragmentation by 30%.
Discussion
The present data substantiate our previous postulates that in cases of breast DIC the zonal increase of type V collagen contribute to the assembly of a "non-permissive" micro-environment for tumor cells, antagonist to other local permissive substrates. It is therefore conceivable that the spatio-temporal derangement of stromal components may actively modulate neoplastic cell behavior and metastatic propensity, thus contributing to the selection and development of more or less malignant tumor phenotypes.