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Virology Journal
Published in: Virology Journal 1/2011

Open Access 01-12-2011 | Research

Tridimensional model structure and patterns of molecular evolution of Pepino mosaic virus TGBp3 protein

Authors: Beata Hasiów-Jaroszewska, Anna Czerwoniec, Henryk Pospieszny, Santiago F Elena

Published in: Virology Journal | Issue 1/2011

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Abstract

Background

Pepino mosaic virus (PepMV) is considered one of the most dangerous pathogens infecting tomatoes worldwide. The virus is highly diverse and four distinct genotypes, as well as inter-strain recombinants, have already been described. The isolates display a wide range on symptoms on infected plant species, ranging from mild mosaic to severe necrosis. However, little is known about the mechanisms and pattern of PepMV molecular evolution and about the role of individual proteins in host-pathogen interactions.

Methods

The nucleotide sequences of the triple gene block 3 (TGB3) from PepMV isolates varying in symptomatology and geographic origin have been analyzed. The modes and patterns of molecular evolution of the TGBp3 protein were investigated by evaluating the selective constraints to which particular amino acid residues have been subjected during the course of diversification. The tridimensional structure of TGBp3 protein has been modeled de novo using the Rosetta algorithm. The correlation between symptoms development and location of specific amino acids residues was analyzed.

Results

The results have shown that TGBp3 has been evolving mainly under the action of purifying selection operating on several amino acid sites, thus highlighting its functional role during PepMV infection. Interestingly, amino acid 67, which has been previously shown to be a necrosis determinant, was found to be under positive selection.

Conclusions

Identification of diverse selection events in TGB3p3 will help unraveling its biological functions and is essential to an understanding of the evolutionary constraints exerted on the Potexvirus genome. The estimated tridimensional structure of TGBp3 will serve as a platform for further sequence, structural and function analysis and will stimulate new experimental advances.
Appendix
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Metadata
Title
Tridimensional model structure and patterns of molecular evolution of Pepino mosaic virus TGBp3 protein
Authors
Beata Hasiów-Jaroszewska
Anna Czerwoniec
Henryk Pospieszny
Santiago F Elena
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2011
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-8-318

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