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Virology Journal
Published in: Virology Journal 1/2011

Open Access 01-12-2011 | Short report

A highly sensitive detection for foot-and-mouth disease virus by gold nanopariticle improved immuno-PCR

Authors: Yao-zhong Ding, Yong-sheng Liu, Jian-hua Zhou, Hao-tai Chen, Gang Wei, Li-na Ma, Jie Zhang

Published in: Virology Journal | Issue 1/2011

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Abstract

Backgroud

Foot-and-mouth disease (FMD) is one of the most contagious of all artiodactyl animal diseases, and its infection has an obvious ability to spread over long distances and to contribute to epidemics in FMD-free areas. A highly sensitive and specific method is required to detect FMDV. In this study, we evaluated the usefulness of a bio-barcode assay (BCA) technique for detecting clinical samples of FMDV.

Methods

Highly sensitive gold nanopariticle (GNP) improved immuno-PCR (GNP-IPCR) which derived from the bio-barcode assay (BCA) was designed for the detection of FMDV. The target viral particles were captured by a polyclonal antibody coated on ELISA microplate, followed by adding GNP which was dually modified with oligonucleotides and a FMDV specific monoclonal antibody (MAb) 1D11 to form a sandwiched immune complex. After the formation of immuno-complex, the signal DNA was released by heating, and consequently characterized by PCR and real time PCR.

Results

The detection limit of GNP-PCR could reach to 10 fg/ml purified FMDV particles, and the assay can detect clinical samples of FMDV with highly sensitivity, while detect limit of conventional ELISA is 100 ng/ml in this study.

Conclusion

GNP-IPCR may provide a highly sensitive method for the detection of FMDV.
Appendix
Available only for authorised users
Literature
1.
Knowles NJ, Samuel AR: Molcular epidemiology of foot-and- mouth disease virus. Virus Res. 2003, 91: 65-80. 10.1016/S0168-1702(02)00260-5.CrossRefPubMed
2.
Robson KJH, Harris TJR, Brown F: An assessment by competition hybridisation of the sequence homology between the RNAs of the seven serotypes of FMDV. J Gen Virol. 1977, 37: 271-276. 10.1099/0022-1317-37-2-271.CrossRefPubMed
3.
Davies G: The foot and mouth disease(FMD) epidemic in the United Kingdom 2001. J Comp Immunol Microbiol Infect Dis. 2002, 25: 331-343. 10.1016/S0147-9571(02)00030-9.CrossRef
4.
Thomson GR, Vosloo W, Bastos ADS: Foot-and-mouth disease in wildlife. Virus Res. 2003, 91: 145-161. 10.1016/S0168-1702(02)00263-0.CrossRefPubMed
5.
Alexandersen S, Zhang Z, Donaldson AI, Garland AJ: The pathogenesis and diagnosis of foot-and-mouth disease. J Comp Pathol. 2003, 129: 1-36. 10.1016/S0021-9975(03)00041-0.CrossRefPubMed
6.
Verheyden B, Lauwers S, Rombaut B: Quantitative RT-PCR ELISA to determine the amount and ratio of positive- and negative-strand viral RNA synthesis and the effect of guanidine in poliovirus infected cells. J Pharm Biomed Anal. 2003, 33: 303-308. 10.1016/S0731-7085(03)00286-3.CrossRefPubMed
7.
Moss A, Hass B: Comparison of the plaque test and reverse transcription nested PCR for detection of FMDV in nasal swabs and probing samples. J Virol Methods. 1999, 80: 59-67. 10.1016/S0166-0934(99)00031-2.CrossRefPubMed
8.
Callahan JD, Brown F, Osorio FA, Sur JH, Kramer E, Long GW, Lubroth J, Ellis SJ, Shoulars KS, Gaffney KL, Rock DL, Nelson WM: Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus. J Am Vet Med Assoc. 2002, 220: 1636-1642. 10.2460/javma.2002.220.1636.CrossRefPubMed
9.
King DP, Ferris NP, Shaw AE, Reid SM, Hutchings GH, Giuffre AC, Robida JM, Callahan JD, Nelson WM, Beckham TR: Detection of foot-and-mouth disease virus: comparative diagnostic sensitivity of two independent real-time reverse transcription-polymerase chain reaction assays. Journal of Veterinary Diagnostic Investigation. 2006, 18: 93-97.CrossRefPubMed
10.
Gu C, Zheng C, Shi L, Zhang Q, Li Y, Lu B, Xiong Y, Qu S, Shao J, Chang H: Plus- and minus-stranded foot-and-mouth disease virus RNA quantified simultaneously using a novel real-time RT-PCR. Virus Genes. 2007, 34: 2461-2469. 10.1007/s11262-006-0019-2.CrossRef
11.
Fosgate GT, Tavornpanich S, Hunter D, Pugh R, Sterle JA, Schumann KR, Eberling AJ, Beckham TR, Martin BM, Clarke NP, Adams LG: Diagnostic specificity of a real-time RT-PCR in cattle for foot-and-mouth disease and swine for foot-and-mouth disease and classical swine fever based on non-invasive specimen collection. Vet. 2008, 132: 158-164.
12.
Nunez JI, Blanco E, Hernandez T, Gomez-Tejedor C, Martin MJ, Dopazo J, Sobrino F: A RT-PCR assay for the differential diagnosis of vesicular viral diseases of swine. J Virol Meth. 1998, 72: 227-235. 10.1016/S0166-0934(98)00032-9.CrossRef
13.
Lomakina NF, Fallacara F, Pacciarini M, Amadori M, Lomakin AI, Timina AM, Shcherbakova LO, Drygin VV: Application of universal primers for identification of foot-and-mouth disease virus and swine vesicular disease virus by PCR and PCR-ELISA. Arch Virol. 2004, 149: 1155-1170. 10.1007/s00705-003-0279-2.CrossRefPubMed
14.
Reid SM, Hutchings GH, Ferris NP, De Clercq K: Diagnosis of foot-and-mouth disease by RT-PCR: evaluation of primers for serotypic characterisation of viral RNA in clinical samples. J Virol Meth. 1999, 83: 113-123. 10.1016/S0166-0934(99)00113-5.CrossRef
15.
Nam JM, Thaxton CS, Mirkin CA: Nanoparticle-based bio-bar codes for the ultrasensitive detection of proteins. Science. 2003, 301: 1884-10.1126/science.1088755.CrossRefPubMed
16.
Bao YP, Wei TF, Lefebvre A, An H, He L, Kunkel GT, Muller UR: Detection of protein analytes via nanoparticle-based bio bar code technology. Anal Chem. 2006, 78: 2055-10.1021/ac051798d.CrossRefPubMed
17.
Stoeva SI, Lee JS, Smith JE, Rosen ST, Mirkin CA: Multiplexed detection of protein cancer markers with biobarcoded nanoparticle probes. J Am Chem Soc. 2006, 128: 8378-10.1021/ja0613106.CrossRefPubMed
18.
Wang XS, Sun Y, Jing S, Ma XM, Zeng Y: Combining gold nanoparticles with real-time immuno-PCR for analysis of HIV p24 antigens. Bioinformatics and Biomedical Engineering. ICBBE. 2007, , The 1st International Conference on: 1198
19.
Chen LY, Wei HP, Guo YC, Cui ZQ, Zhang ZP, Zhang XE: Gold nanoparticle enhanced immuno-PCR for ultrasensitive detection of Hantaan virus nucleocapsid protein. Journal of immunological methods. 2009, 346: 64-70. 10.1016/j.jim.2009.05.007.CrossRefPubMed
20.
Roeder PL, Le Blanc Smith PM: Detection and typing of foot-and-mouth disease virus by enzyme-linked immunosorbent assay: a sensitive, rapid and reliable technique for primary diagnosis. Research in Veterinary Science. 1987, 43: 225-232.PubMed
21.
Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N: Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985, 230: 1350-1354. 10.1126/science.2999980.CrossRefPubMed
22.
Laor O, Torgersen H, Yadin H, Becker Y: Detection of FMDV RNA amplified by the polymerase chain reaction (PCR). Journal of Virological Methods. 1992, 36: 197-207. 10.1016/0166-0934(92)90051-E.CrossRefPubMed
23.
Oleksiewicz MB, Donaldson AI, Alexandersen S: Development of a novel real-time RT-PCR assay for quantitation of foot-and-mouth disease virus in diverse porcine tissues. Journal of Virological Methods. 2001, 92: 23-35. 10.1016/S0166-0934(00)00265-2.CrossRefPubMed
24.
Bao HF, Li D, Guo JH, Lu ZJ, Chen YL, Liu ZX, Liu X T, Xie QG: A highly sensitive and specific multiplex RT-PCR to detect foot-and-mouth disease virus in tissue and food samples. Arch Virol. 2008, 153: 205-209. 10.1007/s00705-007-1082-y.CrossRefPubMed
25.
Huang X, Li Y, Zheng CY: A novel single-cell quantitative real-time RT-PCR method for quantifying foot-and-mouth disease viral RNA. Journal of Virological Methods. 2009, 155: 150-156. 10.1016/j.jviromet.2008.10.007.CrossRefPubMed
26.
Sano T, Smith CL, Cantor CR: Immuno -PCR: very sensitive antigen detection by means of specific conjugates. Science. 1992, 258: 120-122. 10.1126/science.1439758.CrossRefPubMed
27.
Danied MC, Astruc D: Gold nanoparticles: assembly, supram- olecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev. 2004, 104: 293-346. 10.1021/cr030698+.CrossRef
28.
Elghanian R, Storhoff JJ, Mucic RC, Letsinger RL, Mirkin CA: Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. Science. 1997, 277: 1078-1080. 10.1126/science.277.5329.1078.CrossRefPubMed
29.
Mirkin CA, Letsinger RL, Mucic RC, Storhoff JJ: A DNA-based method ofr rationally assembling nanoparticles into macroscopic materials. Nature. 1996, 382: 607-609. 10.1038/382607a0.CrossRefPubMed
30.
Parak WJ, Gerion D, Pellegrino : Biological applications of colloidal nanocrystal. Nanotechnology. 2003, 14: 15-27. 10.1088/0957-4484/14/7/201.CrossRef
31.
Kim EY, Stanton J, Vega RA, Kunstman KJ, Mirkin CA, Wolinsky SM: A real-time PCR-based method for determining the surface coverage of thiol-capped oligonucleotides bound onto gold nanoparticles. Nucleic Acids Res. 2006, 34: e54-10.1093/nar/gkl147.PubMedCentralCrossRefPubMed
32.
Chen HY, Zhuang HS: Real-time immuno-PCR assay for detecting PCBs in soil samples. Anal Bioanal Chem. 2009, 394: 1205-1211. 10.1007/s00216-009-2787-8.CrossRefPubMed
33.
Roth L, Zagon J, Ehlers A, Kroh LW, Broll H: A novel approach for the detection of DNA using immobilized peptide nucleic acid (PDA) probes and signal enhancement by realtime immuno polymerase chain reaction (RT-iPCR). Anal Bioanal Chem. 2009, 394: 529-537. 10.1007/s00216-009-2724-x.CrossRefPubMed
34.
Niemeyer CM, Adler M, Wacker R: Detecting antigens by quantitative immuno-PCR. Natl Protoc. 2007, 2: 1918-1930. 10.1038/nprot.2007.267.CrossRef
Metadata
Title
A highly sensitive detection for foot-and-mouth disease virus by gold nanopariticle improved immuno-PCR
Authors
Yao-zhong Ding
Yong-sheng Liu
Jian-hua Zhou
Hao-tai Chen
Gang Wei
Li-na Ma
Jie Zhang
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2011
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-8-148

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WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

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