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Medical Oncology
Published in: Medical Oncology 10/2016

01-10-2016 | Original Paper

Trichomonas vaginalis: a possible foe to prostate cancer

Authors: Ziwen Zhu, Kristoffer T. Davidson, Andrew Brittingham, Mark R. Wakefield, Qian Bai, Huaping Xiao, Yujiang Fang

Published in: Medical Oncology | Issue 10/2016

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Abstract

Prostate cancer (PCA) is the most common malignancy in men in USA, and the role of Trichomonas vaginalis (T. vag) in the development of PCA is still controversial. Clonogenic assay, PCNA staining, TUNEL staining and caspase-3 activity assay were used to investigate the in vitro role of T. vag in human prostate cancer. We further investigated the possible molecular mechanisms using RT-PCR and immunohistochemical staining. Culture supernatant of T. vag inhibits growth of PC-3 prostate cancer cells, and this correlated with upregulation of p21. Culture supernatant of T. vag induced apoptosis of PC-3 cells, and this correlated with downregulation of Bcl-2. The growth inhibition effect of culture supernatant of T. vag is also demonstrated in another prostate cancer cell line DU145, suggesting that its effect is not specific to one prostate cancer cell line. Culture supernatant of T. vag inhibits growth of prostate cancer by inhibition of proliferation and promotion of apoptosis. Such a study might be helpful to address the association between PCA and infection of T. vag.
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Metadata
Title
Trichomonas vaginalis: a possible foe to prostate cancer
Authors
Ziwen Zhu
Kristoffer T. Davidson
Andrew Brittingham
Mark R. Wakefield
Qian Bai
Huaping Xiao
Yujiang Fang
Publication date
01-10-2016
Publisher
Springer US
Published in
Medical Oncology / Issue 10/2016
Print ISSN: 1357-0560
Electronic ISSN: 1559-131X
DOI
https://doi.org/10.1007/s12032-016-0832-y

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