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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 6/2018

01-12-2018 | Research Article

Tri-modal In vivo Imaging of Pancreatic Islets Transplanted Subcutaneously in Mice

Authors: Sayuan Liang, Karim Louchami, Bryan Holvoet, Rein Verbeke, Christophe M. Deroose, Bella Manshian, Stefaan J. Soenen, Ine Lentacker, Uwe Himmelreich

Published in: Molecular Imaging and Biology | Issue 6/2018

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Abstract

Purpose

Transplantation of pancreatic islets (PIs) is a promising therapeutic approach for type 1 diabetes. The main obstacle for this strategy is that the outcome of islet engraftment depends on the engraftment site. It was our aim to develop a strategy for using non-invasive imaging techniques to assess the location and fate of transplanted PIs longitudinally in vivo.

Procedures

In order to overcome the limitations of individual imaging techniques and cross-validate findings by different modalities, we have combined fluorine magnetic resonance imaging (F-19 MRI), fluorescence imaging (FLI), and bioluminescent imaging (BLI) for studying subcutaneously transplanted PIs and beta cell-like cells (INS-1E cell line) in vivo. We optimized the transduction (using lentiviral vectors) and labeling procedures (using perfluoro crown ether nanoparticles with a fluorescence dye) for PIs and INS-1E cell imaging.

Results

The feasibility of using the proposed imaging methods for PI assessment was demonstrated both in vitro and in vivo. Our data suggested that F-19 MRI is suitable for high-resolution localization of transplanted cells and PIs; FLI is essential for confirmation of contrast localization by histology; and BLI is a reliable method to assess cell viability and survival after transplantation. No significant side effects on cell viability and function have been observed.

Conclusions

The proposed tri-modal imaging platform is a valuable approach for the assessment of engrafted PIs in vivo. It is potentially suitable for comparing different transplantation sites and evaluating novel strategies for improving PI transplantation technique in the future.
Appendix
Available only for authorised users
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Metadata
Title
Tri-modal In vivo Imaging of Pancreatic Islets Transplanted Subcutaneously in Mice
Authors
Sayuan Liang
Karim Louchami
Bryan Holvoet
Rein Verbeke
Christophe M. Deroose
Bella Manshian
Stefaan J. Soenen
Ine Lentacker
Uwe Himmelreich
Publication date
01-12-2018
Publisher
Springer International Publishing
Published in
Molecular Imaging and Biology / Issue 6/2018
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-018-1192-0

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