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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 6/2018

01-12-2018 | Research Article

PET Imaging Reveals Brain Metabolic Changes in Adolescent Rats Following Chronic Escalating Morphine Administration

Authors: Qing Chen, Haifeng Hou, Jin Feng, Xiaohui Zhang, Yao Chen, Jing Wang, Jianfeng Ji, Xiao He, Hao Wu, Hong Zhang

Published in: Molecular Imaging and Biology | Issue 6/2018

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Abstract

Purpose

Non-medical use of prescription opioids, especially among adolescents, has been substantially increased in recent years. However, the neuromechanism remains largely unexplored. In the present study, we aimed to investigate the brain metabolic changes in adolescent rats following chronic escalating morphine administration using positron emission tomography (PET).

Procedures

2-Deoxy-2-[18F]Fluoro-d-glucose ([18F]FDG) microPET imaging was performed, and statistical parametric mapping (SPM) was used for image analysis. Glucose transporter 3 (Glut-3), dopamine D2 receptor (D2R), and Mμ-opioid receptor (μ-OR) were used for immunostaining analysis.

Results

Cerebral glucose metabolism was increased in the corpus callosum (CC) and right retrosplenial dysgranular cortex (rRSD), while it was decreased in the right ventral pallidum (rVP). The expressions of Glut-3, D2R, and μ-OR were increased in CC and rRSD, while they were decreased in rVP. Furthermore, glucose metabolism and Glut-3 expression were positively correlated with the expressions of D2R or μ-OR in CC, rRSD, and rVP.

Conclusions

[18F]FDG microPET brain imaging study in combination with immunohistological investigation revealed that CC, rRSD, and rVP were specifically involved in opioid dependence in adolescents. Our findings provided valuable insights into the neuromechanism of adolescent addiction of prescription opioids and might have important implications for the development of prevention and intervention approaches.
Literature
1.
Lake S, Milloy MJ, Dong H, Hayashi K, Wood E, Kerr T, DeBeck K (2016) Initiation into prescription opioid injection and associated trends in heroin use among people who use illicit drugs. Drug Alcohol Depen 169:73–79CrossRef
2.
Compton WM, Boyle M, Wargo E (2015) Prescription opioid abuse: problems and responses. Prev Med 80:5–9CrossRef
3.
Boyd CJ, McCabe SE, Cranford JA, Young A (2006) Adolescents' motivations to abuse prescription medications. Pediatrics 6:2472–2480CrossRef
4.
Kalivas PW, O'Brien C (2008) Drug addiction as a pathology of staged neuroplasticity. Neuropsychopharmacology 33:166–180CrossRef
5.
Silva-Rodríguez J, García-Varela L, López-Arias E, Domínguez-Prado I, Cortés J, Pardo-Montero J, Fernández-Ferreiro A, Ruibal Á, Sobrino T, Aguiar P (2016) Impact of benzodiazepines on brain FDG-PET quantification after single-dose and chronic administration in rats. Nucl Med Biol 43:827–834CrossRef
6.
Fowler JS, Volkow ND, Kassed CA, Chang L (2007) Imaging the addicted human brain. Sci Pract Perspect 3:4–16CrossRef
7.
Henry PK, Murnane KS, Votaw JR, Howell LL (2010) Acute brain metabolic effects of cocaine in rhesus monkeys with a history of cocaine use. Brain Imaging Behav 4:212–219CrossRef
8.
Salmanzadeh H, Azizi H, Semnanian S (2017) Adolescent chronic escalating morphine administration induces long lasting changes in tolerance and dependence to morphine in rats. Physiol Behav 174:191–196CrossRef
9.
Zhu Y, Du R, Zhu Y et al (2013) PET mapping of neurofunctional changes in a post-traumatic stress disorder model. J Nucl Med 57:1474–1477CrossRef
10.
Xi W, Su D, Nie B, Yu Y, Shan B, Chen Q, Tian M, Zhang H (2013) 18F-FDG PET study reveals brain functional changes during attention in rats. J Nucl Med 54:1969–1973CrossRef
11.
Nie B, Chen K, Zhao S, Liu J, Gu X, Yao Q, Hui J, Zhang Z, Teng G, Zhao C, Shan B (2013) A rat brain MRI template with digital stereotaxic atlas of fine anatomical delineations in Paxinos space and its automated application in voxel-wise analysis. Hum Brain Mapp 34:1306–1318CrossRef
12.
Paxinos G, Watson C (2007) The rat brain in stereotaxic coordinates, 6th ed. San Diego: Academic Press
13.
Volkow ND, Fowler JS, Wang GJ (2003) The addicted human brain: insights from imaging studies. J Clin Invest 111:1444–1451CrossRef
14.
Edwards TJ, Sherr EH, Barkovich AJ, Richards LJ (2014) Clinical, genetic and imaging findings identify new causes for corpus callosum development syndromes. Brain 137:1579–1613CrossRef
15.
Vargas WM, Bengston L, Gilpin NW, Whitcomb BW, Richardson HN (2014) Alcohol binge drinking during adolescence or dependence during adulthood reduces prefrontal myelin in male rats. J Neurosci 34:14777–14782CrossRef
16.
Bi Y, Yuan K, Feng D, Xing L, Li Y, Wang H, Yu D, Xue T, Jin C, Qin W, Tian J (2015) Disrupted inter-hemispheric functional and structural coupling in internet addiction adolescents. Psychiatry Res 234:157–163CrossRef
17.
Lin F, Wu G, Zhu L, Lei H (2013) Heavy smokers show abnormal microstructural integrity in the anterior corpus callosum: a diffusion tensor imaging study with tract-based spatial statistics. Drug Alcohol Depen 129:82–87CrossRef
18.
Van Groen T, Wyss JM (1992) Connections of the retrosplenial dysgranular cortex in the rat. J Comp Neurol 315:200–216CrossRef
19.
Zhang Y, Gong J, Xie C, Ye EM, Jin X, Song H, Yang Z, Shao Y (2015) Alterations in brain connectivity in three sub-regions of the anterior cingulate cortex in heroin-dependent individuals: evidence from resting state fMRI. Neuroscience 284:998–1010CrossRef
20.
Vann SD, Aggleton JP, Maguire EA (2009) What does the retrosplenial cortex do? Nat Rev Neurosci 10:792–802CrossRef
21.
Radwanska A, Debowska W, Liguz-Lecznar M, Brzezicka A, Kossut M, Cybulska-Klosowicz A (2010) Involvement of retrosplenial cortex in classical conditioning. Behav Brain Res 214:231–239CrossRef
22.
Canales JJ (2013) Deficient plasticity in the hippocampus and the spiral of addiction: focus on adult neurogenesis. Curr Top Behav Neurosci 15:293–312CrossRef
23.
Mickiewicz A, Dallimore J, Napier TC (2009) The ventral pallidum is critically involved in the development and expression of morphine-induced sensitization. Neuropsychopharmacology 34:874–886CrossRef
24.
Hubner CB, Koob GF (1990) The ventral pallidum plays a role in mediating cocaine and heroin self-administration in the rat. Brain Res 508:20–29CrossRef
25.
Febo M, Segarra AC, Nair G, Schmidt K, Duong TQ, Ferris CF (2005) The neural consequences of repeated cocaine exposure revealed by functional MRI in awake rats. Neuropsychopharmacology 30:936–943CrossRef
26.
Richard JM, Ambroggi F, Janak PH, Fields HL (2016) Ventral Pallidum neurons encode incentive value and promote cue-elicited instrumental actions. Neuron 90:1165–1173CrossRef
27.
Vannucci SJ (1994) Developmental expression of GLUT1 and GLUT3 glucose transporters in rat brain. J Neurochem 62:240CrossRef
28.
Piepponen TP, Kivastik T, Katajamäki J et al (1997) Involvement of opioid mu 1 receptors in morphine-induced conditioned place preference in rats. Pharmacol Biochem Behav 58:275–279CrossRef
Metadata
Title
PET Imaging Reveals Brain Metabolic Changes in Adolescent Rats Following Chronic Escalating Morphine Administration
Authors
Qing Chen
Haifeng Hou
Jin Feng
Xiaohui Zhang
Yao Chen
Jing Wang
Jianfeng Ji
Xiao He
Hao Wu
Hong Zhang
Publication date
01-12-2018
Publisher
Springer International Publishing
Published in
Molecular Imaging and Biology / Issue 6/2018
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-018-1188-9

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