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Archives of Gynecology and Obstetrics

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01-05-2015 | General Gynecology

Transplantation of endothelial progenitor cells for improving placental perfusion in preeclamptic rats

Authors: Jianwen Zhu, Xiangwei Cheng, Qianhua Wang, Yan Zhou, Fang Wang, Li Zou

Published in: Archives of Gynecology and Obstetrics | Issue 5/2015

Abstract

Objective

Effective treatments for preeclampsia are currently unavailable. As endothelial progenitor cell-transplantation may improve ischemia, it is an important undertaking to study the role of endothelial progenitor cells for improving the symptom of preeclampsia.

Method

Physiological and pathological changes in foetal rats and pregnant rats were monitored. Endothelial progenitor cells were isolated from the peripheral blood of normal rats and labelled by DiI. Endothelial progenitor cells were transplanted into the placenta of preeclampsia-like rats. Fluorescence microscopy was used to observe the differentiation of transplanted endothelial progenitor cells. Western blotting was used to observe the expression of nestin, an index of brain hypoxia in foetal rats.

Result

The rats suffered from abdominal aortic constriction and NG-nitro-l-arginine methyl ester injection (group F). The proteinuria and blood pressure of pregnant rats in group F increased on the 13th day of pregnancy. The proteinuria and blood pressure of group F was higher than in other groups of rats. The weight of foetal rats and foetal heads significantly decreased in group F compared with other groups. Typical pathological changes of preeclampsia were observed in the placental tissue of group F. In preeclampsia-like rats, transplantation of endothelial progenitor cells led to an increase in placenta angiogenesis. The expression of nestin weakened in endothelial progenitor cell-transplanted rats compared with the non-transplantation group. After EPCs transplantation, physiological parameters in the preeclampsia-like rats were significantly decreased.

Conclusion

Endothelial progenitor cells transplantation could improve preeclampsia-like symptom in rats and endothelial progenitor cell-transplantation relieves intrauterine hypoxia in brain tissues of foetal rats to a certain extent.

Report of the National High Blood Pressure Education (2000) Program Working Group on High Blood Pressure in Pregnancy. Am J Obstet Gynecol 183:S1–S22CrossRef

Sibai BM (2003) Diagnosis and management of gestational hypertension and preeclampsia. Obstet Gynecol 102:181–192CrossRefPubMed

Ness RB, Roberts JM (1996) Heterogeneous causes constituting the single syndrome of preeclampsia: a hypothesis and its implications. Am J Obstet Gynecol 175:1365–1370CrossRefPubMed

Zampetaki A, Kirton JP, Xu Q (2008) Vascular repair by endothelial progenitor cells. Cardiovasc Res 78:413–421CrossRefPubMed

Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, Witzenbichler B, Schatteman G, Isner JM (1997) Isolation of putative progenitor endothelial cells for angiogenesis. Science 275:964–967CrossRefPubMed

Hill JM, Zalos G, Halcox JP, Schenke WH, Waclawiw MA, Quyyumi AA, Finkel T (2003) Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. N Engl J Med 348:593–600CrossRefPubMed

Hauschka SD, Konigsberg IR (1966) The influence of collagen on the development of muscle clones. Proc Natl Acad Sci USA 55:119–126CrossRefPubMedCentralPubMed

Kalka C, Masuda H, Takahashi T, Kalka-Moll WM, Silver M, Kearney M, Li T, Isner JM, Asahara T (2000) Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Proc Natl Acad Sci USA 97:3422–3427CrossRefPubMedCentralPubMed

Nusken KD, Dotsch J, Rauh M, Rascher W, Schneider H (2008) Uteroplacental insufficiency after bilateral uterine artery ligation in the rat: impact on postnatal glucose and lipid metabolism and evidence for metabolic programming of the offspring by sham operation. Endocrinology 149:1056–1063CrossRefPubMed

10.

Tate MD, Deng YM, Jones JE, Anderson GP, Brooks AG, Reading PC (2009) Neutrophils ameliorate lung injury and the development of severe disease during influenza infection. J Immunol 183:7441–7450CrossRefPubMed

11.

Hwang HS, Cho NH, Maeng YS, Kang MH, Park YW, Kim YH (2007) Differential expression of nestin in normal and pre-eclamptic human placentas. Acta Obstet Gynecol Scand 86:909–914CrossRefPubMed

12.

Raijmakers MT, Peters WH, Steegers EA, Poston L (2004) NAD(P)H oxidase associated superoxide production in human placenta from normotensive and pre-eclamptic women. Placenta 25(Suppl A):S85–S89CrossRefPubMed

13.

Kanayama N, Tsujimura R, She L, Maehara K, Terao T (1997) Cold-induced stress stimulates the sympathetic nervous system, causing hypertension and proteinuria in rats. J Hypertens 15:383–389CrossRefPubMed

14.

Granger JP, LaMarca BB, Cockrell K, Sedeek M, Balzi C, Chandler D, Bennett W (2006) Reduced uterine perfusion pressure (RUPP) model for studying cardiovascular-renal dysfunction in response to placental ischemia. Methods Mol Med 122:383–392PubMed

15.

Yallampalli C, Garfield RE (1993) Inhibition of nitric oxide synthesis in rats during pregnancy produces signs similar to those of preeclampsia. Am J Obstet Gynecol 169:1316–1320CrossRefPubMed

16.

Chambers JC, Fusi L, Malik IS, Haskard DO, De Swiet M, Kooner JS (2001) Association of maternal endothelial dysfunction with preeclampsia. JAMA 285:1607–1612CrossRefPubMed

17.

de Oliveira LG, Karumanchi A, Sass N (2010) Preeclampsia: oxidative stress, inflammation and endothelial dysfunction. Rev Bras Ginecol Obstet 32:609–616CrossRefPubMed

18.

Maslov AY, Barone TA, Plunkett RJ, Pruitt SC (2004) Neural stem cell detection, characterization, and age-related changes in the subventricular zone of mice. J Neurosci 24:1726–1733CrossRefPubMed

19.

Lendahl U, Zimmerman LB, McKay RD (1990) CNS stem cells express a new class of intermediate filament protein. Cell 60:585–595CrossRefPubMed

20.

Rehman J, Li J, Orschell CM, March KL (2003) Peripheral blood “endothelial progenitor cells” are derived from monocyte/macrophages and secrete angiogenic growth factors. Circulation 107:1164–1169CrossRefPubMed

Title: Transplantation of endothelial progenitor cells for improving placental perfusion in preeclamptic rats
Authors: Jianwen Zhu
Xiangwei Cheng
Qianhua Wang
Yan Zhou
Fang Wang
Li Zou
Publication date: 01-05-2015
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 5/2015
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-014-3522-z

At a glance: The STEP trials

Springer Medicine

Transplantation of endothelial progenitor cells for improving placental perfusion in preeclamptic rats

Abstract

Objective

Method

Result

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Method

Result

Conclusion

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