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Archives of Gynecology and Obstetrics

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01-08-2012 | Reproductive Medicine

Transfer of blastocysts derived from frozen-thawed cleavage stage embryos improved ongoing pregnancy

Authors: Maryam Eftekhar, Abbas Aflatoonian, Farnaz Mohammadian, Nasim Tabibnejad

Published in: Archives of Gynecology and Obstetrics | Issue 2/2012

Abstract

Purpose

The aim of our study was to compare the transfer of embryos that are cryopreserved in cleavage stage after thawing with the transfer of embryos after thawing and culture in sequential media until blastocyst formation.

Methods

In this prospective clinical study, we have evaluated 134 cycles of ART treatment for infertility. Frozen embryos were thawed and then cultured in sequential media until blastocyst stage in blastocyst group and were compared with thawed embryos in cleavage stage group.

Results

Implantation rate was significantly higher in blastocyst group (30 %) compared to cleavage group (17 %). No statistical differences were reported in chemical and clinical pregnancy rates between groups. Ongoing pregnancy rate was significantly higher in blastocyst group compared to cleavage group (42.9 vs. 24.6 %).

Conclusions

Our results indicated that blastocyst formation after thawing of cleavage stage embryos is a good predictor for embryo viability and pregnancy outcome.

Raju GAR, Prakash GJ, Krishna KM, Madan K (2009) Vitrification of human early cavitating and deflated expanded blastocysts: clinical outcome of 474 cycles. J Assist Reprod Genet 26:523–529PubMedCrossRef

Behr B, Pool T, Milki A, Moore D, Gebhardt J, Dasig D (1999) Preliminary clinical experience with human blastocyst development in vitro without co-culture. Hum Reprod 14:454–457PubMedCrossRef

Alikani M, Calderon G, Tomkin G, Garrisi J, Kokot M, Cohen J (2000) Cleavage anomalies in early human embryos and survival after prolonged culture in vitro. Hum Reprod 15:2634–2643. doi:10.1093/humrep/15.12.2634 PubMedCrossRef

Papanikolaou EG, Camus M, Kolibianakis EM, Van Landuyt L, Van Steirteghem A, Devroey P (2006) In vitro fertilization with single blastocyst-stage versus single cleavage-stage embryos. N Engl J Med 16:1139–1146CrossRef

Blake D, Proctor M, Johnson N, Olive D (2005) Cleavage stage versus blastocyst stage embryo transfer in assisted conception. Cochrane Database Syst Rev 4:CD002118

De los Santos M, Mercader A, Galan A, Albert C, Romero J, Pellicer A (2003) Implantation rates after two, three, or five days of embryo culture. Placenta 24:S13–S19PubMedCrossRef

Ebner T, Yaman C, Moser M, Sommergruber M, Jesacher K, Tews G (2001) A prospective study on oocyte survival rate after ICSI: influence of injection technique and morphological features. J Assist Reprod Genetics 18:623–628

Johnson N, Blake D, Farquhar C (2007) Blastocyst or cleavage-stage embryo transfer? Best Practice Res Clin Obstet Gynaecol 21(1):21–40CrossRef

Moragianni VA, Cohen JD, Smith SE, Schinfeld JS, Somkuti SG, Lee A, Barmat LI (2010) Outcomes of day-1, day-3, and blastocyst cryopreserved embryo transfers. Fertil Steril 93:1353–1355PubMedCrossRef

10.

Papanikolaou EG, D’haeseleer E, Verheyen G, Van de Velde H, Camus M, Van Steirteghem A, Devroey P, Tournaye H (2005) Live birth rate is significantly higher after blastocyst transfer than after cleavage-stage embryo transfer when at least four embryos are available on day 3 of embryo culture. A randomized prospective study. Hum Reprod 20:3198–3203PubMedCrossRef

11.

Aflatoonian A, Oskouian H, Ahmadi S, Oskouian L (2010) Can fresh embryo transfers be replaced by cryopreserved-thawed embryo transfers in assisted reproductive cycles? A randomized controlled trial. J Assist Reprod Genetics 27:357–363

12.

Rienzi L, Ubaldi F, Iacobelli M, Ferrero S, Minasi MG, Martinez F, Tesarik J, Greco E (2002) Day 3 embryo transfer with combined evaluation at the pronuclear and cleavage stages compares favourably with day 5 blastocyst transfer. Hum Reprod 17:1852–1855PubMedCrossRef

13.

Stoop D, Van Landuyt L, Van den Abbeel E, Camus M, Verheyen G, Devroey P (2011) Should a single blastocyst transfer policy be a clinical decision or should it depend on the embryological evaluation on day 3? Reprod Biol Endocrinol 9:60PubMedCrossRef

14.

Papanikolaou EG, Kolibianakis EM, Tournaye H, Venetis CA, Fatemi H, Tarlatzis B, Devroey P (2008) Live birth rates after transfer of equal number of blastocysts or cleavage-stage embryos in IVF. A systematic review and meta-analysis. Hum Reprod 23:91–99PubMedCrossRef

15.

Khalaf Y, El Toukhy T, Coomarasamy A, Kamal A, Bolton V, Braude P (2008) Selective single blastocyst transfer reduces the multiple pregnancy rate and increases pregnancy rates: a pre and postintervention study. BJOG 115:385–390PubMedCrossRef

16.

Zhu D, Zhang J, Cao S, Zhang J, Heng BC, Huang M, Ling X, Duan T, Tong GQ (2011) Vitrified-warmed blastocyst transfer cycles yield higher pregnancy and implantation rates compared with fresh blastocyst transfer cycles: time for a new embryo transfer strategy? Fertil Steril 95:1691–1695PubMedCrossRef

17.

Ménézo YJ (2004) Blastocyst freezing. Eur J Obstet Gynecol Reprod Biol 115:S12–S15PubMedCrossRef

18.

Milki AA, Hinckley MD, Gebhardt J, Dasig D, Westphal LM, Behr B (2002) Accuracy of day 3 criteria for selecting the best embryos. Fertil Steril 77:1191–1195PubMedCrossRef

19.

Pantos K, Stefanidis K, Pappas K, Kokkinopoulos P, Petroutsou K, Kokkali G, Stavrou D, Tzigounis V (2001) Cryopreservation of embryos, blastocysts, and pregnancy rates of blastocysts derived from frozen-thawed embryos and frozen-thawed blastocysts. J Assist Reprod Genet 18:579–582PubMedCrossRef

20.

Wang YA, Chapman M, Costello M, Sullivan EA (2010) Better perinatal outcomes following transfer of fresh blastocysts and blastocysts cultured from thawed cleavage embryos: a population-based study. Hum Reprod 25:1536–1542. doi:10.1093/humrep/deq067 PubMedCrossRef

21.

Joshi BV, Banker MR, Patel PM, Shah PB (2010) Transfer of human frozen-thawed embryos with further cleavage during culture increases pregnancy rates. J Hum Reprod Sci 3:76–79PubMedCrossRef

22.

Criniti A, Thyer A, Chow G, Lin P, Klein N, Soules M (2005) Elective single blastocyst transfer reduces twin rates without compromising pregnancy rates. Fertil Steril 84:1613–1619PubMedCrossRef

23.

Gardner DK, Schoolcraft WB, Wagley L, Schlenker T, Stevens J, Hesla J (1998) A prospective randomized trial of blastocyst culture and transfer in in vitro fertilization. Hum Reprod 13:3434–3440PubMedCrossRef

24.

Gardner DK, Vella P, Lane M, Wagley L, Schlenker T, Schoolcraft WB (1998) Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers. Fertil Steril 69:84–88PubMedCrossRef

Title: Transfer of blastocysts derived from frozen-thawed cleavage stage embryos improved ongoing pregnancy
Authors: Maryam Eftekhar
Abbas Aflatoonian
Farnaz Mohammadian
Nasim Tabibnejad
Publication date: 01-08-2012
Publisher: Springer-Verlag
Published in: Archives of Gynecology and Obstetrics / Issue 2/2012
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-012-2303-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Transfer of blastocysts derived from frozen-thawed cleavage stage embryos improved ongoing pregnancy

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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