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Published in: Gut Pathogens 1/2021

01-12-2021 | Antibiotic | Genome Report

Comparative genomics of two Shewanella xiamenensis strains isolated from a pilgrim before and during travels to the Hajj

Authors: Thongpan Leangapichart, Linda Hadjadj, Philippe Gautret, Jean-Marc Rolain

Published in: Gut Pathogens | Issue 1/2021

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Abstract

Background

Shewanella xiamenensis has been reported in water environment and in patients and can act as the originator of oxacillinase in gram-negative bacteria. In order to assess genome plasticity and its functional properties related diarrhea symptoms in pilgrim, comparisons of draft genome sequences of the two isolates were conducted with other closely related genomes.

Results

We isolated S. xiamenensis 111B and 111D strains from a pilgrim before travels to the Hajj and during travels with diarrhea symptom, respectively. Whole-genome sequencing showed that draft genome size of 111B strain was 5,008,191 bp, containing 49 kb of a putative plasmid. The genome size of 111D was 4,964,295 bp containing 225 kb of a putative plasmid that shared the backbone sequences with the hospital wastewater strain T17. Comparatively, two Hajj strains are identical at 97.3% identity and 98.7% coverage. They are closely related to river water strain, AS58 by SNPs analysis. Notably, a novel blaOXA-48 allele blaOXA-547 was identified in 111D, sharing 99.5% identity with blaOXA-546 and blaOXA-894. Multiple copies of virulence specific genes, such as capsular polysaccharide biosynthesis, O-antigen and lasB (vibriolysin related gene) have been identified specifically in 111D, but absent in 111B strain.

Conclusions

The whole genome sequences of S. xiamenensis strain 111B and 111D, including comparative genomic analysis, highlight here the potential for virulence factors that might be related to the cause of diarrhea in humans and also indicate the possible acquisition of pathogenic bacteria, including antibiotic resistance genes or plasmids during the Hajj.
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Metadata
Title
Comparative genomics of two Shewanella xiamenensis strains isolated from a pilgrim before and during travels to the Hajj
Authors
Thongpan Leangapichart
Linda Hadjadj
Philippe Gautret
Jean-Marc Rolain
Publication date
01-12-2021
Publisher
BioMed Central
Keyword
Antibiotic
Published in
Gut Pathogens / Issue 1/2021
Electronic ISSN: 1757-4749
DOI
https://doi.org/10.1186/s13099-021-00404-w

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