Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Respiratory Research
Published in: Respiratory Research 1/2016

Open Access 01-12-2016 | Letter to the Editor

Human lung fibroblasts may modulate dendritic cell phenotype and function: results from a pilot in vitro study

Authors: Olivia Freynet, Joëlle Marchal-Sommé, Francette Jean-Louis, Arnaud Mailleux, Bruno Crestani, Paul Soler, Laurence Michel

Published in: Respiratory Research | Issue 1/2016

Login to get access

Abstract

In human lung fibrotic lesions, fibroblasts were shown to be closely associated with immature dendritic cell (DC) accumulation. The aim of the present pilot study was to characterize the role of pulmonary fibroblasts on DC phenotype and function, using co-culture of lung fibroblasts from patients with idiopathic pulmonary fibrosis (IPF) and from control patients, with a DC cell line MUTZ-3. We observed that co-culture of lung control and IPF fibroblasts with DCs reduced the expression of specific DC markers and down-regulated their T-cell stimulatory activity. This suggests that pulmonary fibroblasts might sustain chronic inflammation in the fibrotic lung by maintaining in situ a pool of immature DCs.
Literature
1.
Lipscomb MF, Masten BJ. Dendritic cells: immune regulators in health and disease. Physiol Rev. 2002;82:97–130.CrossRefPubMed
2.
Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Kambouchner M, Valeyre D, et al. Dendritic cells accumulate in human fibrotic interstitial lung disease. Am J Respir Crit Care Med. 2007;176:1007–14.CrossRefPubMed
3.
Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, et al. Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis. J Immunol. 2006;176:5735–9.CrossRefPubMed
4.
5.
Svensson M, Kaye PM. Stromal-cell regulation of dendritic-cell differentiation and function. Trends Immunol. 2006;27:580–7.CrossRefPubMed
6.
Berthier R, Rizzitelli A, Martinon-Ego C, Laharie AM, Collin V, et al. Fibroblasts inhibit the production of interleukin-12p70 by murine dendritic cells. Immunology. 2003;108:391–400.CrossRefPubMedPubMedCentral
7.
Butler M, Ng CY, van Heel DA, Lombardi G, Lechler R, et al. Modulation of dendritic cell phenotype and function in an in vitro model of the intestinal epithelium. Eur J Immunol. 2006;36:864–74.CrossRefPubMed
8.
Zhang M, Tang H, Guo Z, An H, Zhu X, et al. Splenic stroma drives mature dendritic cells to differentiate into regulatory dendritic cells. Nat Immunol. 2004;5:1124–33.CrossRefPubMed
9.
Guironnet G, Dezutter-Dambuyant C, Gaudillere A, Marechal S, Schmitt D, et al. Phenotypic and functional outcome of human monocytes or monocyte-derived dendritic cells in a dermal equivalent. J Invest Dermatol. 2001;116:933–9.CrossRefPubMed
10.
Mollah ZU, Aiba S, Manome H, Yoshino Y, Tagami H. Cord blood CD34+ cells differentiate into dermal dendritic cells in co-culture with cutaneous fibroblasts or stromal cells. J Invest Dermatol. 2002;118:450–60.CrossRefPubMed
11.
Kitamura H, Cambier S, Somanath S, Barker T, Minagawa S, et al. Mouse and human lung fibroblasts regulate dendritic cell trafficking, airway inflammation, and fibrosis through integrin αvβ8-mediated activation of TGF-β. J Clin Invest. 2011;121:2863–75.CrossRefPubMedPubMedCentral
12.
Haniffa MA, Wang XN, Holtick U, Rae M, Isaacs JD, et al. Adult human fibroblasts are potent immunoregulatory cells and functionally equivalent to mesenchymal stem cells. J Immunol. 2007;179:1595–604.CrossRefPubMed
13.
Saalbach A, Klein C, Sleeman J, Sack U, Kauer F, et al. Dermal fibroblasts induce maturation of dendritic cells. J Immunol. 2007;178:4966–74.CrossRefPubMed
14.
Saalbach A, Klein C, Schirmer C, Briest W, Anderegg U, et al. Dermal fibroblasts promote the migration of dendritic cells. J Invest Dermatol. 2010;130:444–54.CrossRefPubMed
15.
van Helden SF, van Leeuwen FN, Figdor CG. Human and murine model cell lines for dendritic cell biology evaluated. Immunol Lett. 2008;117:191–7.CrossRefPubMed
16.
Marchand-Adam S, Fabre A, Mailleux A, Marchal J, Quesnel C, et al. Defect of pro-hepatocyte growth factor activation by fibroblasts in idiopathic pulmonary fibrosis. Am J Respir Crit Care Med. 2006;174:58–66.CrossRefPubMed
17.
Larsson K, Lindstedt M, Borrebaeck CA. Functional and transcriptional profiling of MUTZ-3, a myeloid cell line acting as a model for dendritic cells. Immunology. 2006;117:156–66.CrossRefPubMedPubMedCentral
18.
Gabrilovich D. Mechanisms and functional significance of tumour-induced dendritic-cell defects. Nat Rev Immunol. 2004;4:941–52.CrossRefPubMed
19.
Li MO, Wan YY, Sanjabi S, Robertson AK, Flavell RA. Transforming growth factor-beta regulation of immune responses. Annu Rev Immunol. 2006;24:99–146.CrossRefPubMed
20.
Masterson AJ, Sombroek CC, De Gruijl TD, Graus YM, van der Vliet HJ, et al. MUTZ-3, a human cell line model for the cytokine-induced differentiation of dendritic cells from CD34+ precursors. Blood. 2002;100:701–703.21.CrossRefPubMed
21.
Santegoets SJ, Schreurs MW, Masterson AJ, Liu YP, Goletz S, et al. In vitro priming of tumor-specific cytotoxic T lymphocytes using allogeneic dendritic cells derived from the human MUTZ-3 cell line. Cancer Immunol Immunother. 2006;2006(55):1480–90.CrossRef
22.
Lundberg K, Albrekt AS, Nelissen I, Santegoets S, de Gruijl TD, et al. Transcriptional profiling of human dendritic cell populations and models--unique profiles of in vitro dendritic cells and implications on functionality and applicability. PLoS One. 2013;8(1):e52875.CrossRefPubMedPubMedCentral
23.
Li Q, Guo Z, Xu X, Xia S, Cao X. Pulmonary stromal cells induce the generation of regulatory DC attenuating T-cell-mediated lung inflammation. Eur J Immunol. 2008;38:2751–61.CrossRefPubMed
24.
Uccelli A, Moretta L, Pistoia V. Immunoregulatory function of mesenchymal stem cells. Eur J Immunol. 2006;36:2566–73.CrossRefPubMed
Metadata
Title
Human lung fibroblasts may modulate dendritic cell phenotype and function: results from a pilot in vitro study
Authors
Olivia Freynet
Joëlle Marchal-Sommé
Francette Jean-Louis
Arnaud Mailleux
Bruno Crestani
Paul Soler
Laurence Michel
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Respiratory Research / Issue 1/2016
Electronic ISSN: 1465-993X
DOI
https://doi.org/10.1186/s12931-016-0345-4

Other articles of this Issue 1/2016

Respiratory Research 1/2016 Go to the issue

Research

Ajulemic acid exerts potent anti-fibrotic effect during the fibrogenic phase of bleomycin lung

Research

Electronic cigarettes: a survey of perceived patient use and attitudes among members of the British thoracic oncology group

Letter to the Editor

Airway smooth muscle NOX4 is upregulated and modulates ROS generation in COPD

Research

Increased expression of TROP2 in airway basal cells potentially contributes to airway remodeling in chronic obstructive pulmonary disease

Research

Identification of vitamin D sensitive pathways during lung development

Editorial

An imperfect “PAST” Lessons learned from the National Review of Asthma Deaths (NRAD) UK
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits