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01-04-2020 | Toxoplasmosis | Original Article

Murine leukocytes stimulation by Toxoplasma gondii antigens induces high level of IFN-γ production

Authors: Ali Fattahi-Bafghi, Farzaneh Mirzaei, Aghdas Mirjalili, Mohammad Kazemi Arababadi, Roghiyeh Faridnia, Mahdi Fakhar, Hamed Kalani

Published in: Comparative Clinical Pathology | Issue 2/2020

Abstract

Toxoplasma gondii (T. gondii) is an obligate intracellular parasitic protozoan, infecting a wide range of warm-blooded vertebrates. It is important for T. gondii to balance the response between cellular and humoral immune system for various aspects of its own survival. The aim of this study was to determine the level of IFN-γ and IL-4 production by murine peritoneal leukocytes stimulated with T. gondii lysate antigens (TLAs), excretory/secretory antigens (ESAs), and a combination of them (TLAs:ESAs). TLAs, ESAs, and TLA + ESA, considered as the experimental groups, were prepared and used to stimulate murine peritoneal leukocytes. The level of the IFN-γ and IL-4 secreted by the stimulated leukocytes was measured. In addition, untreated and phytohemagglutinin-treated groups were considered as negative and positive controls, respectively. As compared to the negative control, the level of IL-4 was lower in each of the experimental groups (P < 0.05); however, no statistically significant difference was observed between the positive control and each of the experimental groups (P > 0.05). Moreover, there was a significant difference for the level of IFN-γ between each of the experimental groups and both negative and positive controls (P < 0.05). The findings showed that the antigens of T. gondii were able to increase the IFN-γ production and decrease the level of IL-4 production.

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Title: Murine leukocytes stimulation by Toxoplasma gondii antigens induces high level of IFN-γ production
Authors: Ali Fattahi-Bafghi
Farzaneh Mirzaei
Aghdas Mirjalili
Mohammad Kazemi Arababadi
Roghiyeh Faridnia
Mahdi Fakhar
Hamed Kalani
Publication date: 01-04-2020
Publisher: Springer London
Keyword: Toxoplasmosis
Published in: Comparative Clinical Pathology / Issue 2/2020
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-020-03091-2

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Murine leukocytes stimulation by Toxoplasma gondii antigens induces high level of IFN-γ production

Abstract

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Abstract

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