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Environmental Health and Preventive Medicine
Published in: Environmental Health and Preventive Medicine 1/2017

Open Access 01-12-2017 | Research article

Total arsenic and speciation analysis of saliva and urine samples from individuals living in a chronic arsenicosis area in China

Authors: Dapeng Wang, Yasuyo Shimoda, Sanxiang Wang, Zhenghui Wang, Jian Liu, Xing Liu, Huanyu Jin, Fenfang Gao, Jian Tong, Kenzo Yamanaka, Jie Zhang, Yan An

Published in: Environmental Health and Preventive Medicine | Issue 1/2017

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Abstract

Background

It is generally acknowledged that the determination of harmful chemical compounds excreted into saliva is useful for assessing their exposure levels. The aim of the present study was to compare the total arsenic and its species in saliva and urine samples collected from the people residing in an arsenic-contaminated area of China and to further verify the feasibility of using salivary arsenic as a new biomarker of arsenic exposure.

Methods

Total arsenic and speciation analyses in urine and saliva samples among 70 residents exposed to arsenic from drinking water in Shanxi, China were carried out by high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP/MS).

Results

The result showed that, total arsenic concentration in saliva was relatively lower than in urine samples, but it existed a strong positive correlation with total urinary arsenic, drinking water arsenic and different skin lesions. For arsenic metabolism analyses, AsIII, AsV, MMA, and DMA were detected in all of the urine samples with the dominating species of DMA (73.2%). Different with urinary arsenic species, most arsenic species in saliva were not methylated. The major species in saliva was iAs (AsIII + AsV, 76.18%), followed by DMA (13.08%) and MMA (9.13%). And the primary methylation index (PMI), second methylation index (SMI) and proportion of the four different species (AsIII, AsV, MMA, and DMA) in saliva showed no significant positive relationship with that of in urine.

Conclusions

These findings indicated saliva may be used as a useful tool for biological monitoring of total arsenic exposure in the crowd rather than an efficient tool for assessing arsenic metabolism in human body after exposed to arsenic.
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Metadata
Title
Total arsenic and speciation analysis of saliva and urine samples from individuals living in a chronic arsenicosis area in China
Authors
Dapeng Wang
Yasuyo Shimoda
Sanxiang Wang
Zhenghui Wang
Jian Liu
Xing Liu
Huanyu Jin
Fenfang Gao
Jian Tong
Kenzo Yamanaka
Jie Zhang
Yan An
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Environmental Health and Preventive Medicine / Issue 1/2017
Print ISSN: 1342-078X
Electronic ISSN: 1347-4715
DOI
https://doi.org/10.1186/s12199-017-0652-5

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