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Archives of Gynecology and Obstetrics

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01-04-2017 | Gynecologic Oncology

The role of the SDF-1/ CXCR7 axis on the growth and invasion ability of endometrial cancer cells

Authors: Hong-qin Gu, Zhen-bo Zhang, Jia-wen Zhang, Qian-qian Wang, Xiao-wei Xi, Yin-yan He

Published in: Archives of Gynecology and Obstetrics | Issue 4/2017

Abstract

Purpose

Stroma-derived factor-1 (SDF-1) and its receptor C-X-C chemokine receptor-4 (CXCR4) are involved in human endometrial carcinoma (EC) progression. CXCR7 is another important receptor of SDF-1 and has a higher affinity with SDF-1 compared with that of CXCR4. This paper aims to study the effects of the SDF-1/CXCR7 axis on the growth and invasion ability of EC cells.

Methods

CXCR7 expression was evaluated by quantitative RT-PCR, immunohistochemistry, immunocytochemistry and Western blotting in EC cell lines and 30 cases of primary EC tissue from patients. EC cell line proliferation and migration were assessed following knockdown of CXCR7 by MTT and transwell assays.

Results

The results showed that CXCR7 was highly expressed at both mRNA and protein levels in the EC cells and tissue. siCXCR7 effectively silenced CXCR7 in Ishikawa and AN3CA cells. Treatment with 17β-oestradiol (17β-E2) significantly increased the levels of CXCR7 and SDF-1 in Con, siCon and siCXCR7 treated Ishikawa. siCXCR7 persistently inhibited CXCR7 expression, even in cells treated with 17β-E2. Moreover, in vitro functional analyses, silencing CXCR7 resulted in decreased proliferation in Ishikawa and AN3CA cells. Treatment with 17β-E2 and SDF-1 significantly promoted the growth and migration in siCon treated Ishikawa and AN3CA. Interestingly, in response to 17β-E2 and SDF-1 stimulation, siCXCR7 continuously inhibited the growth and invasion of Ishikawa and AN3CA cells.

Conclusion

Our results indicate that SDF-1/CXCR7 plays a positive role in the proliferation and invasion of EC cells. CXCR7 inhibition treatment may provide a promising strategy for anti-tumour therapy for EC.

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Title: The role of the SDF-1/ CXCR7 axis on the growth and invasion ability of endometrial cancer cells
Authors: Hong-qin Gu
Zhen-bo Zhang
Jia-wen Zhang
Qian-qian Wang
Xiao-wei Xi
Yin-yan He
Publication date: 01-04-2017
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 4/2017
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-017-4308-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The role of the SDF-1/ CXCR7 axis on the growth and invasion ability of endometrial cancer cells

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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