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01-04-2017 | Gynecologic Endocrinology and Reproductive Medicine

Expression of AKT1 along with AKT2 in granulosa-lutein cells of hyperandrogenic PCOS patients

Authors: Saeid Nekoonam, Mohammad Naji, Maryam Shabani Nashtaei, Keywan Mortezaee, Morteza Koruji, Leili Safdarian, Fardin Amidi

Published in: Archives of Gynecology and Obstetrics | Issue 4/2017

Abstract

Purpose

AKTs have a pivotal role in the granulosa-lutein cell (GC) proliferation and folliculogenesis, and there is a reciprocal feedback between AKT with androgen. Therefore, we aimed to evaluate the role of AKTs in GCs of hyperandrogenic (+HA) PCOS cases.

Method

There were three groups: control, +HA PCOS and –HA (non-hyperandrogenic) PCOS. All groups were subjected to GnRH antagonist protocol for stimulation of ovulation. Follicular fluid was aspirated from large follicles, and GCs were isolated using cell strainer method. AKT1, AKT2, AKT3, and androgen receptor (AR) mRNA expressions were analyzed with quantitative real-time PCR (qRT-PCR), and total-AKT and p-AKT (Ser⁴⁷³ & Thr³⁰⁸) were investigated using western blotting.

Results

There were high levels of AKT1, AKT2, and AR mRNA expressions and high levels of p-AKT protein expression in the +HA PCOS group (p ≤ 0.05). There was a direct positive correlation between free testosterone (FT) and total testosterone (TT) with the levels of AKT1, AKT2, and p-AKT (Ser⁴⁷³), and also between FT with the levels of AR.

Conclusion

High expressions of AKT1 and AKT2 through possible relation with androgen may cause GCs dysfunction in the +HA PCOS patients.

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Title: Expression of AKT1 along with AKT2 in granulosa-lutein cells of hyperandrogenic PCOS patients
Authors: Saeid Nekoonam
Mohammad Naji
Maryam Shabani Nashtaei
Keywan Mortezaee
Morteza Koruji
Leili Safdarian
Fardin Amidi
Publication date: 01-04-2017
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 4/2017
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-017-4317-9

At a glance: The STEP trials

Springer Medicine

Expression of AKT1 along with AKT2 in granulosa-lutein cells of hyperandrogenic PCOS patients

Abstract

Purpose

Method

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Method

Results

Conclusion

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