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Journal of Assisted Reproduction and Genetics

Published in:

01-11-2011 | Genetics

The prevalence of common CFTR mutations in Iranian infertile men with non-CAVD obstructive azoospermia by using ARMS PCR techniques

Authors: Kyumars Safinejad, Mojtaba Darbouy, Sayed Mahdi Kalantar, Sirus Zeinali, Reza Mirfakhraie, Leila Yadegar, Masoud Houshmand

Published in: Journal of Assisted Reproduction and Genetics | Issue 11/2011

Abstract

Purpose

To evaluate five common cystic fibrosis trans-membrane conductance regulator (CFTR) mutations (ΔF508, G542X, R117H, W1282X and N1303K) in the Iranian infertile men with noncongenital absence of vas deferens (CAVD) obstructive azoospermia.

Methods

The common CFTR gene mutations were tested on blood samples from 53 infertile men with non-CAVD obstructive azoospermia and 50 normal men as control individuals. Genomic DNA is extracted from the whole blood and the common CFTR mutations have been detected by the amplification refractory mutation system (ARMS) techniques.

Results

The common CFTR mutations were found positive in 5/53)9.43%(for ΔF508 and 4/53)7.55%(for G542X mutation of all patients tested. Also, no CFTR mutations were detected in the normal men.

Conclusion

The common CFTR mutations were detected in 9/53(17%) infertile men with non-CAVD obstructive azoospermia. Pre-treatment CFTR mutation analysis remains critical to distinguish cystic fibrosis (CF) genotypes for men with non CAVD obstructive azoospermia.

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Title: The prevalence of common CFTR mutations in Iranian infertile men with non-CAVD obstructive azoospermia by using ARMS PCR techniques
Authors: Kyumars Safinejad
Mojtaba Darbouy
Sayed Mahdi Kalantar
Sirus Zeinali
Reza Mirfakhraie
Leila Yadegar
Masoud Houshmand
Publication date: 01-11-2011
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 11/2011
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9632-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The prevalence of common CFTR mutations in Iranian infertile men with non-CAVD obstructive azoospermia by using ARMS PCR techniques

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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